A synthetic biscoumarin suppresses lung cancer cell proliferation and induces cell apoptosis by increasing expression of RIP1.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Ruixue Wang, Hongyi Xie, Xi Wang, Yingqi Liu, Zhengquan Su, Zhaoguang Zheng
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引用次数: 0

Abstract

Coumarin has a variety of biological activities and widely exists in plants. Biscoumarin, derived from coumarin, their synthetic methods and bioactivities of biscoumarins is the hotspot of the current research. In this study, we evaluated for the first time the anticancer of a synthetic biscoumarin (3,3'-(4-chlorophenyl)methylene)bis(4-hydroxy-2H-chromen-2-one, C3) on lung cancer cells and explored the related mechanism. C3 was simply prepared by 4-hydroxycoumarin and 4-chlorobenzaldehyde under ethanol. The structure of C3 was elucidated by various spectroscopic analyses. The antiproliferation effect of C3 was evaluated by the cell counting kit-8 assay. Cell cycle and apoptosis analysis were detected by flow cytometry. The expression of correlated proteins was determined using Western blotting. The result showed that C3 displayed a strong cytostatic effect on Lewis lung cancer (LLC) cells. C3 inhibited the proliferation of LLC cells, and induced G2/M phase cell cycle arrest. In addition, C3 possessed a significant reduction on cell apoptosis by increasing of RIP1 expression. Our data showed that C3 suppresses lung cancer cell proliferation and induces cell apoptosis, which is possibly involved with the RIP1.

合成的双香豆素通过增加RIP1的表达抑制肺癌细胞增殖,诱导细胞凋亡。
香豆素具有多种生物活性,广泛存在于植物中。双香豆素是由香豆素衍生而来,其合成方法和生物活性是目前研究的热点。本研究首次评价了合成双香豆素(3,3′-(4-氯苯基)亚甲基)双(4-羟基- 2h - chromen2 -one, C3)对肺癌细胞的抗癌作用,并探讨了其作用机制。以4-羟基香豆素和4-氯苯甲醛为原料,在乙醇条件下制备C3。通过各种光谱分析对C3的结构进行了分析。采用细胞计数试剂盒-8法评价C3的抗增殖作用。流式细胞术检测细胞周期和凋亡分析。Western blotting检测相关蛋白的表达。结果表明,C3对Lewis肺癌(LLC)细胞有较强的细胞抑制作用。C3抑制LLC细胞增殖,诱导G2/M期细胞周期阻滞。此外,C3通过增加RIP1的表达显著降低细胞凋亡。我们的数据显示C3抑制肺癌细胞增殖并诱导细胞凋亡,这可能与RIP1有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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