Combined effects of voluntary running and liraglutide on glucose homeostasis, fatty acid composition of brown adipose tissue phospholipids, and white adipose tissue browning in db/db mice.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Ruili Yin, Yan Ma, Ning Zhang, Longyan Yang, Dong Zhao
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引用次数: 5

Abstract

There is a potential therapeutic application targeting brown adipose tissue (BAT). Either voluntary running or liraglutide increases the thermogenesis of BAT in type 2 diabetes mellitus, but their combined effect is not yet clarified. Male leptin receptor-deficient db/db diabetic mice (n = 24) were randomly divided into voluntary running, liraglutide, voluntary running + liraglutide, and control groups (n = 6/group). Normal male C57 mice were the negative control (n = 6). Fasting blood glucose was monitored every week, plasma insulin and lipid profiles were analyzed, and thermogenic protein expression in BAT and white adipose tissue (WAT) were analyzed by the western blot. A total of 128 metabolites associated with phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, and ceramides were targeted in BAT. Compared to the control group, voluntary running or liraglutide treatment significantly lowered the blood glucose and increased the insulin level; the combined group showed a better effect than liraglutide alone. Hence, the combined treatment showed an enhanced hypoglycemic effect. Uncoupling protein 1 (UCP1) and OXPHOS protein expression in BAT and UCP1 in WAT were significantly increased after exercise training and liraglutide treatment. However, BAT metabolomics showed that compared to the control mice, nine fatty acids increased in the exercise group, six increased in the liraglutide group, and only three increased in the combined group. These results may suggest a higher hypoglycemic effect and the activation of BAT and WAT browning in the combined group.

自主跑步和利拉鲁肽对db/db小鼠葡萄糖稳态、棕色脂肪组织磷脂脂肪酸组成和白色脂肪组织褐变的联合影响。
有一个潜在的治疗应用针对棕色脂肪组织(BAT)。自主跑步或利拉鲁肽均可增加2型糖尿病患者的BAT产热,但其联合作用尚未明确。雄性瘦素受体缺陷db/db糖尿病小鼠(n = 24)随机分为自主跑步组、利拉鲁肽组、自主跑步+利拉鲁肽组和对照组(n = 6/组)。正常雄性C57小鼠为阴性对照(n = 6),每周监测空腹血糖,分析血浆胰岛素和血脂水平,用western blot法分析BAT和白色脂肪组织(WAT)中产热蛋白的表达。总共128种与磷脂酰胆碱、磷脂酰乙醇胺、鞘磷脂和神经酰胺相关的代谢物在BAT中被靶向。与对照组相比,自愿跑步或利拉鲁肽治疗显著降低了血糖,增加了胰岛素水平;联合用药组疗效优于单用利拉鲁肽。因此,联合治疗显示出增强的降糖效果。运动训练和利拉鲁肽治疗后,BAT和WAT中Uncoupling protein 1 (UCP1)和OXPHOS蛋白的表达均显著升高。然而,BAT代谢组学显示,与对照组小鼠相比,运动组有9种脂肪酸增加,利拉鲁肽组有6种脂肪酸增加,联合组只有3种脂肪酸增加。这些结果可能表明联合组有更高的降糖效果和BAT和WAT褐变的激活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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