Towards better models for studying human adipocytes in vitro.

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM
Fabiana Baganha, Ruby Schipper, Carolina E Hagberg
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引用次数: 2

Abstract

With obesity and its comorbidities continuing to rise, we urgently need to improve our understanding of what mechanisms trigger the white adipose tissue to become dysfunctional in response to over-feeding. The recent invent of 3D culturing models has produced several noteworthy protocols for differentiating unilocular adipocytes in vitro, promising to revolutionize the obesity research field by providing more representative adipose tissue models for such mechanistic studies. In parallel, these 3D models provide important insights to how profoundly the microenvironment influences adipocyte differentiation and morphology. This commentary highlights some of the most recent 3D models, including human unilocular vascularized adipocyte spheroids (HUVASs), developed by our lab. We discuss recent developments in the field, provide further insights to the importance of the microvasculature for adipocyte maturation, and summarize what challenges remain to be solved before we can achieve a culture model that fully recapitulates all aspects of human white adipocyte biology in vitro. Taken together, the commentary highlights important recent advances regarding 3D adipocyte culturing and underlines the many advantages these models provide over traditional 2D cultures, with the aim of convincing more laboratories to switch to 3D models.

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为体外研究人类脂肪细胞建立更好的模型。
随着肥胖及其合并症的持续增加,我们迫切需要提高我们对触发白色脂肪组织因过度喂养而功能失调的机制的理解。最近发明的3D培养模型已经产生了几个值得注意的体外单眼脂肪细胞分化方案,有望通过为此类机制研究提供更具代表性的脂肪组织模型来彻底改变肥胖研究领域。同时,这些3D模型为微环境对脂肪细胞分化和形态的影响提供了重要的见解。这篇评论重点介绍了一些最新的3D模型,包括我们实验室开发的人类单眼血管化脂肪细胞球体(HUVASs)。我们讨论了该领域的最新发展,进一步阐述了微血管系统对脂肪细胞成熟的重要性,并总结了在我们能够实现一个完全概括体外人类白色脂肪细胞生物学的各个方面的培养模型之前仍有待解决的挑战。综上所述,评论强调了3D脂肪细胞培养的重要最新进展,并强调了这些模型比传统2D培养提供的许多优势,目的是说服更多的实验室转向3D模型。
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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
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