Amphiregulin Supplementation During Pig Oocyte In Vitro Maturation Enhances Subsequent Development of Cloned Embryos by Promoting Cumulus Cell Proliferation.

IF 1.2 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cellular reprogramming Pub Date : 2022-08-01 Epub Date: 2022-07-20 DOI:10.1089/cell.2022.0015

Xianjun Zhang, Huaxing Zhao, Yanan Li, Yuxing Zhang, Yalin Liang, Junsong Shi, Rong Zhou, Linjun Hong, Gengyuan Cai, Zhenfang Wu, Zicong Li

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引用次数: 2

Abstract

The oocyte in vitro maturation (IVM) technique is important in animal husbandry, biomedicine, and human-assisted reproduction. However, the developmental potential of in vitro matured oocytes is usually lower than that of in vivo matured (IVVM) oocytes. Amphiregulin (AREG) is an EGF-like growth factor that plays critical roles in the maturation and development of mammalian oocytes. This study investigated the effects of AREG supplementation during pig oocyte IVM on the subsequent development of cloned embryos. The addition of AREG to pig oocyte IVM medium improved the developmental competence of treated oocyte-derived cloned embryos by enhancing the expansion and proliferation of cumulus cells (CCs) during IVM. The positive effect of AREG on enhancing the quality of IVVM pig oocytes might be due to the activation of proliferation-related pathways in CCs by acting on the AREG receptor. The present study provides an AREG treatment-based method to improve the developmental competence of cloned pig embryos.

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猪卵母细胞体外成熟过程中补充双调节蛋白通过促进卵丘细胞增殖促进克隆胚胎的后续发育。

卵母细胞体外成熟技术在畜牧业、生物医学和人类辅助生殖等领域具有重要的应用价值。然而，体外成熟卵母细胞的发育潜力通常低于体内成熟卵母细胞。双调节蛋白(AREG)是一种egf样生长因子，在哺乳动物卵母细胞的成熟和发育中起关键作用。本研究探讨了猪卵母细胞体外受精过程中添加AREG对克隆胚胎后续发育的影响。在猪卵母细胞IVM培养基中添加AREG，通过促进卵母细胞积云细胞(CCs)的扩增和增殖，提高了卵母细胞克隆胚胎的发育能力。AREG对提高IVVM猪卵母细胞质量的积极作用可能是通过作用于AREG受体激活cc中增殖相关通路。本研究提供了一种基于AREG处理的方法来提高克隆猪胚胎的发育能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular reprogramming CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.50

自引率

6.20%

发文量

审稿时长

3 months

期刊介绍： Cellular Reprogramming is the premier journal dedicated to providing new insights on the etiology, development, and potential treatment of various diseases through reprogramming cellular mechanisms. The Journal delivers information on cutting-edge techniques and the latest high-quality research and discoveries that are transforming biomedical research. Cellular Reprogramming coverage includes: Somatic cell nuclear transfer and reprogramming in early embryos Embryonic stem cells Nuclear transfer stem cells (stem cells derived from nuclear transfer embryos) Generation of induced pluripotent stem (iPS) cells and/or potential for cell-based therapies Epigenetics Adult stem cells and pluripotency.