Beneficial role of naringin against methotrexate-induced injury to rat testes: biochemical and ultrastructural analyses.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Redox Report Pub Date : 2022-12-01 DOI:10.1080/13510002.2022.2101832

Hany Elsawy, Abdullah M Alzahrani, Manal Alfwuaires, Ashraf M Abdel-Moneim, Mahmoud Khalil

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引用次数: 3

Abstract

Background: Methotrexate (MTX) is a commonly used chemotherapeutic drug that has adverse toxic effects on germ cells. Naringin (NG) is a natural flavanone glycoside, with different phytotherapeutic applications, and its possible protective effects against MTX-induced testicular tissue damage were investigated in this study.

Methods: Low and high doses of NG (40 and 80 mg/kg/day) were given for 10 days by intraperitoneal (i.p.) injection and MTX (20 mg/kg i.p.) was given at the 4th day of the experiment, with or without NG in rats.

Results: The obtained results showed that exposure to MTX increased malondialdehyde (MDA) levels and nitric oxide (NO) production compared with the control. In the meantime, MTX depleted catalse (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), and reduced glutathione (GSH) in the testicular tissue. Further, serum testosterone levels were significantly decreased in the MTX group. NG significantly counteracted the aforementioned effects of MTX; however, NG80 was more effective in restoring SOD, GR, MDA and NO. Interestingly, NG80 achieved a better improvement in the ultrastructural pattern of the testicular cells in MTX-exposed rats.

Conclusion: These results indicated, for the first time, that NG could be a potential candidate therapy against MTX-reprotoxic impacts.

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柚皮苷对甲氨蝶呤致大鼠睾丸损伤的有益作用:生化和超微结构分析。

背景:甲氨蝶呤(MTX)是一种常用的化疗药物，对生殖细胞有不良的毒性作用。柚皮苷(Naringin, NG)是一种天然的黄酮苷类化合物，具有多种植物治疗用途，本研究探讨了其对mtx致睾丸组织损伤的保护作用。方法:大鼠腹腔注射低、高剂量NG(40、80 mg/kg/d) 10 d，实验第4天分别给予MTX (20 mg/kg/ p)，加NG或不加NG。结果:得到的结果表明，与对照组相比，暴露于MTX增加丙二醛(MDA)水平和一氧化氮(NO)的产生。同时MTX减少睾丸组织中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPx)和还原性谷胱甘肽(GSH)。此外，MTX组血清睾酮水平显著降低。NG显著抵消了MTX的上述作用;NG80对SOD、GR、MDA和NO的恢复效果较好。有趣的是，NG80对mtx暴露大鼠睾丸细胞的超微结构模式有更好的改善。结论:这些结果首次表明，NG可能是抗甲氨蝶呤生殖毒性影响的潜在候选疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.