Screening of Crucial Differentially-Methylated/Expressed Genes for Alzheimer's Disease.

IF 2.7 4区医学 Q2 CLINICAL NEUROLOGY

American Journal of Alzheimers Disease and Other Dementias Pub Date : 2022-01-01 DOI:10.1177/15333175221116220

Haiyuan Qiu, Qiuyan Weng

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引用次数: 0

Abstract

Background: We aimed to make an integrated analysis of published transcriptome and DNA methylation dataset to ascertain the key differentially methylated and differentially expressed genes for Alzherimer's disease (AD). Methods: Two gene expression microarrays and 1 gene methylation microarray were downloaded for identification of differentially expressed genes and differentially methylated genes. Then, we used various biological information databases to annotate the functions of the differentially-methylated/expressed genes, and screen out key genes and important signaling pathways. Finally, we validate the differentially-methylated/expressed genes in the additional online datasets and in blood from AD patients.Results: A total of 8 hub hypomethylated-high expression genes were obtained, including Rac family small GTPase 2, FGR proto-oncogene, Src family tyrosine kinase, LYN proto-oncogene, Src family tyrosine kinase, protein kinase C delta, myosin IF, integrin subunit alpha 5, semaphorin 4D, and growth arrest specific protein 7. Some enriched signaling pathways of hypomethylated-high expression genes were identified, including regulation of actin cytoskeleton, chemokine signaling pathway, Fc gamma R-mediated phagocytosis, and axon guidance. Conclusion: Differentially-methylated/expressed genes are likely to be associated with AD.

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筛查阿尔茨海默病的关键差异甲基化/表达基因

研究背景我们旨在对已发表的转录组和 DNA 甲基化数据集进行综合分析，以确定阿尔茨海默病（AD）的关键差异甲基化基因和差异表达基因。研究方法下载两个基因表达微阵列和一个基因甲基化微阵列，以确定差异表达基因和差异甲基化基因。然后，利用各种生物信息数据库注释差异甲基化/表达基因的功能，筛选出关键基因和重要信号通路。最后，我们在其他在线数据集和AD患者血液中验证了差异甲基化/表达基因：结果：共获得了8个中枢低甲基化高表达基因，包括Rac家族小GTP酶2、FGR原癌基因、Src家族酪氨酸激酶、LYN原癌基因、Src家族酪氨酸激酶、蛋白激酶C delta、肌球蛋白IF、整合素亚基α5、semaphorin 4D和生长停滞特异性蛋白7。研究还发现了低甲基化高表达基因的一些丰富信号通路，包括肌动蛋白细胞骨架调控、趋化因子信号通路、Fc gamma R介导的吞噬作用和轴突导向。结论不同甲基化/表达的基因可能与AD有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Alzheimers Disease and Other Dementias GERIATRICS & GERONTOLOGY-CLINICAL NEUROLOGY

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： American Journal of Alzheimer''s Disease and other Dementias® (AJADD) is for professionals on the frontlines of Alzheimer''s care, dementia, and clinical depression--especially physicians, nurses, psychiatrists, administrators, and other healthcare specialists who manage patients with dementias and their families. This journal is a member of the Committee on Publication Ethics (COPE).