Stability of Some Biochemical Parameters in Sheep and Goat Serum Stored at -20℃.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-11-21 eCollection Date: 2022-01-01 DOI:10.2147/VMRR.S391254

Yoseph Cherinet Megerssa

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引用次数: 0

Abstract

Background: In Veterinary Medicine biochemical investigation of serum is widely used to aid diagnosis and treatment. However, delays usually happen between sampling and analysis. As a result, the serum is stored in refrigerators. In this regard, information on the effects of temperature and storage duration on the stability of the analyte is incomplete in general and its effect in sheep and goat serum is not described. Therefore, the objective of this study was to examine the stability of selected biochemical analytes from sheep and goat serum following storage at -20℃ for 2 months.

Methods: Serum from 20 apparently healthy male 2-2.5 year-old sheep and goats was obtained and aliquots of serum from each sample were kept in three tubes. The first tube is for baseline (T0), which is done within an hour, while the other two (T1 and T2) are stored at -20℃ for 1 and 2 months, respectively. Total protein, albumin, urea, total cholesterol, and triglycerides were assayed.

Results: The results revealed that storage temperature and duration for up to 2 months had no significant effect on any analytes except for urea in goats. The changes in terms of total observed error (TE_o) for total protein; albumin and urea were greater than the acceptable values in both animals.

Conclusion: Thus, further studies are required to assure alteration of analyte at various storage temperatures and duration. In addition, implementation of quality systems to achieve quality targets for analytes with greater TE_o as compared to the established TE_a is needed.

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-20℃保存绵羊和山羊血清中一些生化指标的稳定性

背景:在兽医学中，血清生化检测被广泛用于辅助诊断和治疗。然而，延迟通常发生在采样和分析之间。结果，血清被储存在冰箱里。在这方面，一般来说，关于温度和储存时间对分析物稳定性影响的信息是不完整的，而且它在绵羊和山羊血清中的作用也没有描述。因此，本研究的目的是检测从绵羊和山羊血清中选择的生化分析物在-20℃保存2个月后的稳定性。方法:采集20只2 ~ 2.5岁健康雄性绵羊和山羊的血清，每只血清等份保存在3个试管中。第一管为基线(T0)， 1小时内完成，另外两管(T1和T2)分别在-20℃保存1个月和2个月。检测总蛋白、白蛋白、尿素、总胆固醇和甘油三酯。结果:2个月的贮藏温度和贮存时间对除山羊尿素外的其它分析物无显著影响。总蛋白的总观测误差(TEo)的变化;两种动物的白蛋白和尿素均高于可接受值。结论:因此，需要进一步的研究来确保分析物在不同的储存温度和时间下的变化。此外，需要实施质量体系，以实现与既定TEa相比具有更大TEo的分析物的质量目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.