Resveratrol regulates the inflammation and oxidative stress of granulosa cells in PCOS via targeting TLR2.

IF 2.9 4区 生物学 Q2 BIOPHYSICS
Ben Yuan, Shuhong Luo, Liulian Feng, Junling Wang, Junbiao Mao, Bingbing Luo
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引用次数: 6

Abstract

Polycystic ovary syndrome (PCOS) is featured as a common endocrine disorder in reproductive-aged women, while its pathophysiology is not fully illustrated. This study examined potential actions of resveratrol in PCOS cellular model and explored the underlying interaction between resveratrol and toll-like receptor 2 (TLR2). This study performed the bioinformatics analysis on two microarray datasets (GSE34526 and GSE138518). We found that TLR2 was one of potential hub genes that may be associated with PCOS. Further examination showed that TLR2 was highly expressed in granulosa cells from PCOS group compared with control. The in vitro studies showed that LPS intervention caused an increased expression of TLR2 and the pro-inflammatory mediators, and induced oxidative stress in the granulosa cells, which was concentration-dependently antagonized by resveratrol treatment. TLR2 silence significantly attenuated LPS-induced increase TNF-α, IL-1β, IL-6 and IL-8 expression and oxidative stress of granulosa cells. Furthermore, TLR2 overexpression promoted inflammatory response and oxidative stress in the granulosa cells, which was antagonized by resveratrol treatment. In conclusion, resveratrol could attenuate LPS-induced inflammation and oxidative stress in granulosa cells, and the underlying mechanisms may be related to the inhibitory effect of resveratrol on TLR2 expression in granulosa cells.

Abstract Image

白藜芦醇通过靶向TLR2调控PCOS颗粒细胞的炎症和氧化应激。
多囊卵巢综合征(PCOS)是育龄妇女中一种常见的内分泌疾病,其病理生理机制尚未完全阐明。本研究考察了白藜芦醇在PCOS细胞模型中的潜在作用,并探讨了白藜芦醇与toll样受体2 (TLR2)之间的潜在相互作用。本研究对两个微阵列数据集(GSE34526和GSE138518)进行了生物信息学分析。我们发现TLR2是可能与PCOS相关的潜在枢纽基因之一。进一步检测发现,与对照组相比,PCOS组颗粒细胞中TLR2表达较高。体外研究表明,LPS干预可引起颗粒细胞TLR2及促炎介质表达增加,诱导氧化应激,白藜芦醇可浓度依赖性拮抗氧化应激。TLR2沉默可显著减弱lps诱导的颗粒细胞TNF-α、IL-1β、IL-6、IL-8表达升高及氧化应激。此外,TLR2过表达可促进颗粒细胞的炎症反应和氧化应激,而白藜芦醇可拮抗这一作用。综上所述,白藜芦醇可以减轻lps诱导的颗粒细胞炎症和氧化应激,其机制可能与白藜芦醇抑制颗粒细胞TLR2表达有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
22
审稿时长
6-12 weeks
期刊介绍: The Journal of Bioenergetics and Biomembranes is an international journal devoted to the publication of original research that contributes to fundamental knowledge in the areas of bioenergetics, biomembranes, and transport, including oxidative phosphorylation, photosynthesis, muscle contraction, as well as cellular and systemic metabolism. The timely research in this international journal benefits biophysicists, membrane biologists, cell biologists, biochemists, molecular biologists, physiologists, endocrinologists, and bio-organic chemists.
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