Anisakis spp, DNA detection in paraffin-embedded tissue biopsies recovered from patients with gastritis using real-time PCR in Bushehr, Persian Gulf, Iran

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular and biochemical parasitology Pub Date : 2022-09-01 DOI:10.1016/j.molbiopara.2022.111494

Mohsen Najjari , Seyed Mahmoud Sadjjadi , Hossein Khodadadi , Mohamad Reza Farzaneh , Simonetta Mattiucci

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引用次数: 1

Abstract

Anisakiasis is a zoonotic fish-born parasitic disease caused by anisakid nematodes. Paraffin-embedded blocks containing biopsy samples taken from patients suffering gastritis with unknown causes were investigated by real-time PCR, in the Bushehr region, Iran; where human anisakiasis has not been reported, so far. A total of 50 paraffin-embedded blocks were randomly selected from 250 archived blocks of the patients with gastritis. A SYBER green-based real-time PCR targeting the ITS1 region was developed for the identification of Anisakis genus. An 86 bp partial fragment of the Anisakis spp. ITS1 gene was amplified successfully. A total of 3 out of 50 samples (6 %) had positive amplification in the samples and their pathology reports showed a significant finding of moderate chronic gastritis with or without ulcers. In conclusion, the developed qPCR could be used for detecting Anisakis spp. larval DNA in human biopsy blocks. This study showed the hidden human cases of anisakiasis in the Bushehr for the first time.

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实时荧光定量PCR法检测伊朗布什尔地区胃炎患者石蜡包埋组织活检标本中的异尖线虫(Anisakis spp)

茴香线虫病是由茴香线虫引起的人畜共患鱼类寄生虫病。在伊朗Bushehr地区，用实时荧光定量PCR技术对来自不明原因胃炎患者的活检样本进行了石蜡包埋块检测;到目前为止，还没有人类异长线虫病的报告。从250例存档胃炎患者标本中随机抽取50例石蜡包埋标本。建立了一种针对ITS1区域的SYBER绿色实时PCR方法，用于鉴别异尖线虫属。成功扩增了异尖akis spp. ITS1基因86bp的部分片段。50个样本中有3个(6%)样本中有阳性扩增，他们的病理报告显示有明显的中度慢性胃炎伴或不伴溃疡。结果表明，所建立的qPCR可用于人活检块中异尖线虫幼虫DNA的检测。本研究首次发现了布什尔地区隐藏的人类异烟线虫病病例。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.