Effects of carvacrol on experimental nephrolithiasis in female rats.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnic & Histochemistry Pub Date : 2023-01-01 Epub Date: 2022-11-29 DOI:10.1080/10520295.2022.2147218
Tuncer Kutlu, Mehmet Güvenç
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引用次数: 0

Abstract

We induced experimental nephrolithiasis in female rats using ethylene glycol (EG) and ammonium chloride (AC). We investigated the effects of carvacrol, an essential oil with antioxidant and anti-inflammatory properties, on nephrolithiasis using histopathology, immunohistochemistry and biochemistry. We used 40 female rats divided into four equal groups: control group, administered olive oil; carvacrol group, administered carvacrol in olive oil; nephrolithiasis group, administered EG and AC to induce experimental nephrolithiasis; treatment group with induced nephrolithiasis and administered carvacrol in olive oil. We observed no significant difference in crystal accumulation in the treatment group compared to the nephrolithiasis group. We found a significant reduction in hydropic degeneration of tubules and degree of inflammatory cell infiltration of intertubule areas. We also found a significant reduction in immunohistochemical staining of macrophage- and monocyte-specific antigens. Carvacrol treatment reversed the induced nephrolithiasis, increased malondialdehyde and urea, and decreased levels of glutathione peroxidase and catalase. Although carvacrol did not decrease crystal accumulation, it reduced pathological and biochemical damage, and improved kidney function by lowering the serum urea level.

香芹酚对实验性雌性大鼠肾结石的影响。
我们用乙二醇(EG)和氯化铵(AC)诱导雌性大鼠实验性肾结石。采用组织病理学、免疫组织化学和生物化学方法研究了具有抗氧化和抗炎作用的香芹酚精油对肾结石的作用。我们将40只雌性大鼠分为四组:对照组,给予橄榄油;香芹酚组,给予橄榄油香芹酚;肾结石组,给予EG和AC诱导实验性肾结石;治疗组以诱导性肾结石合并橄榄油香芹酚。我们观察到治疗组与肾结石组在晶体积累方面无显著差异。我们发现小管水变性和小管间区炎症细胞浸润程度显著减少。我们还发现巨噬细胞和单核细胞特异性抗原的免疫组织化学染色显著减少。Carvacrol治疗逆转了诱导的肾结石,增加丙二醛和尿素,降低谷胱甘肽过氧化物酶和过氧化氢酶的水平。虽然carvacrol不能减少结晶的积累,但可以减轻病理和生化损伤,并通过降低血清尿素水平改善肾功能。
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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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