Thymoquinone ameliorates amikacin induced oxidative damage in rat brain tissue.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2023-01-01 Epub Date: 2022-07-11 DOI:10.1080/10520295.2022.2087905

Sedat Bilgiç, Meltem Özgöçmen, Mehmet Kaya Ozer

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引用次数: 3

Abstract

We investigated the potential neuroprotective effects of thymoquinone (TQ) on amikacin (AK) induced oxidative damage in rat brain. We used 21 male rats divided randomly into three equal groups. The control group was injected intraperitoneally (i.p.) with 0.5 ml 0.9% aqueous NaCl and given 1 ml 0.9% aqueous NaCl orally. The AK group was administered 1.2 g/kg aqueous AK i.p. as a single dose on the day 3 of the study. The AK + TQ group was given a single 1.2 g/kg dose of AK i.p. on the day 3 of the study plus 40 mg/kg/day TQ by oral gavage daily. Treatment with TQ increased serum ferritin and decreased serum calcium levels significantly. TQ also decreased NADPH oxidase-2, NADPH oxidase-4, and caspase-3 levels. Decreased malondialdehyde (MDA) levels and increased superoxide dismutase (SOD) and catalase (CAT) activities were detected in the AK + TQ group compared to the AK group. TQ administration inhibited lipid peroxide formation and blocked oxidative reactions, which reduced the MDA level and increased SOD and CAT activities induced by AK. Oxidative damage caused by AK was ameliorated by TQ treatment owing to its antioxidative and anti-apoptotic effects. TQ may be a potential therapeutic agent for reducing the severity of AK induced oxidative damage to the brain.

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百里醌改善阿米卡星诱导的大鼠脑组织氧化损伤。

我们研究了百里醌(TQ)对阿米卡星(AK)引起的大鼠脑氧化损伤的潜在神经保护作用。我们选用21只雄性大鼠，随机分为三组。对照组患者腹腔注射0.9%氯化钠0.5 ml, 0.9%氯化钠1 ml口服。AK组于研究第3天单次给药1.2 g/kg AK水溶液。AK + TQ组在研究第3天给予单次1.2 g/kg剂量的AK，每天口服40 mg/kg/天TQ。TQ治疗可显著提高血清铁蛋白水平，降低血清钙水平。TQ还能降低NADPH氧化酶-2、NADPH氧化酶-4和caspase-3水平。与AK组相比，AK + TQ组丙二醛(MDA)水平降低，超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性升高。TQ抑制脂质过氧化形成，阻断氧化反应，降低MDA水平，提高AK诱导的SOD和CAT活性。由于其抗氧化和抗凋亡作用，TQ处理可改善AK引起的氧化损伤。TQ可能是一种潜在的治疗药物，可以减轻AK诱导的脑氧化损伤的严重程度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.