Determination of UDP-Glucose and UDP-Galactose in Maize by Hydrophilic Interaction Liquid Chromatography and Tandem Mass Spectrometry.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2022-06-28 eCollection Date: 2022-01-01 DOI:10.1155/2022/7015311

Chen Lan, Bing Zhao, Lu Yang, Yusen Zhou, Siyi Guo, Xuebin Zhang, Junli Zhang

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引用次数: 0

Abstract

Nucleotide sugars, the activated forms of monosaccharides, are important intermediates of carbohydrate metabolism in all organisms. Here, we describe a method for the detection and quantification of UDP-glucose and UDP-galactose in maize in order to compare their metabolism in both wild-type and mutated plants. Triple quadrupole operating in a multiple reaction monitoring mode was used to quantify nucleotide sugars. The limits of detection for UDP-glucose and UDP-galactose were 0.50 and 0.70 ng·mL^-1, respectively. The recoveries of the method ranged from 98.3% to 103.6% with the relative standard deviations less than 6.3%. To prove the applicability of this method, we analyzed several sets of maize extracts obtained from different cultivars grown under standardized greenhouse conditions. All the results demonstrated the suitability of the developed method to quantify UDP-glucose and UDP-galactose in maize extracts.

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亲水相互作用液相色谱-串联质谱法测定玉米中udp -葡萄糖和udp -半乳糖。

核苷酸糖是单糖的活化形式，是所有生物体碳水化合物代谢的重要中间体。在这里，我们描述了一种检测和定量玉米中udp -葡萄糖和udp -半乳糖的方法，以便比较它们在野生型和突变植物中的代谢。在多反应监测模式下使用三重四极杆来定量核苷酸糖。检测限分别为0.50 ng·mL-1和0.70 ng·mL-1。加样回收率为98.3% ~ 103.6%，相对标准偏差小于6.3%。为了证明该方法的适用性，我们分析了在标准化温室条件下生长的不同品种的几组玉米提取物。结果表明，该方法适用于玉米提取物中udp -葡萄糖和udp -半乳糖的定量测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.