Hemolysis interference on clinical chemistry tests analyzed on DxC 700 AU (Beckman Coulter®) and kaliemia rendering algorithm

IF 0.4 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Emeline Sanandedji, Julien André, Alexi Lienard, Caroline Hentgen, Alain Barrans
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引用次数: 1

Abstract

The influence of hemolysis was evaluated for 26 clinical chemistry parameters on DxC 700AU (Beckman Coulter®). Ten sample pools were prepared and separated into six aliquots. These aliquots were overloaded with hemolysis in increasing amounts to reach levels equivalent to the maximum hemolysis thresholds H1 (+), H2 (++), H3 (+++) and H4 (++++). Each aliquot is compared to its reference aliquot (not hemolyzed) and a ratio is calculated for each parameter. We proposed that there was a significant difference if, for a given analyte and threshold, more than 20% of the ratios are above the total acceptable limit variability. A significant difference was found for TGP, TGO, cholesterol, creatine kinase (CPK), lactate deshydrogenase (LDH), phosphorus and potassium at H1 (+), chlorine, iron, γ-glutamyltransferase (GGT), and magnesium at H2 (++), amylase and alkaline phosphatase (PAL) at H3 (++++), and prealbumin at H4 (++++). No interference was found until H4 included for uric acid, calcium, creatinine, lipase, glucose, HDL-cholesterol, triglycerides, urea, sodium and immunoglobulins A, G and M. The overestimation of kalemia was calculated as a function of hemolysis, ranging from 0.28 mM +/–0.047 (upper H1 threshold) to 1.37 mM +/–0.126 (upper H4 threshold). Its estimation makes it possible to propose a result rendering algorithm of kalemia according to the hemolysis index. Evaluation of the automates hemolysis indexes is highly recommended for each laboratory. It can allow for some critical parameters the establishment of a decision tree facilitating the result rendering, after clinicobiological consultation.

采用DxC 700 AU (Beckman Coulter®)和血钾绘制算法分析溶血对临床化学试验的干扰
评估溶血对DxC 700AU (Beckman Coulter®)26项临床化学参数的影响。准备了10个样本池,分成6个等份。这些等分体溶血负荷越来越大,达到溶血最大阈值H1(+)、H2(++)、H3(+++)和H4(++++)的水平。将每个成分与其参考成分(未溶血)进行比较,并计算每个参数的比率。我们提出,对于给定的分析物和阈值,如果超过20%的比率高于可接受的总极限可变性,则存在显着差异。TGP、TGO、胆固醇、肌酸激酶(CPK)、乳酸脱氢酶(LDH)、H1(+)磷和钾、氯、铁、γ-谷氨酰转移酶(GGT)、H2(++)镁、H3(++++)淀粉酶和碱性磷酸酶(PAL)、H4(++++)前白蛋白含量差异显著。在H4纳入尿酸、钙、肌酐、脂肪酶、葡萄糖、高密度脂蛋白胆固醇、甘油三酯、尿素、钠和免疫球蛋白A、G和m之前,没有发现干扰。根据溶血功能计算血钾的高估,范围从0.28 mM +/ -0.047 (H1上限)到1.37 mM +/ -0.126 (H4上限)。它的估计使得根据溶血指数提出血钾的结果呈现算法成为可能。强烈建议每个实验室对自动溶血指标进行评估。在临床生物学会诊后,它可以允许一些关键参数建立决策树,促进结果呈现。
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来源期刊
Annales de biologie clinique
Annales de biologie clinique 医学-医学:研究与实验
CiteScore
0.80
自引率
20.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: Multidisciplinary information with direct relevance to everyday practice Annales de Biologie Clinique, the official journal of the French Society of Clinical Biology (SFBC), supports biologists in areas including continuing education, laboratory accreditation and technique validation. With original articles, abstracts and accounts of everyday practice, the journal provides details of advances in knowledge, techniques and equipment, as well as a forum for discussion open to the entire community.
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