Transcriptome analysis of the ink sac and brain tissues from Sepiella inermis: A resource for discovering genes related to the inking of cephalopods

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-08-01 DOI:10.1016/j.margen.2022.100968

Shanshan Zhou , Tao Zhang , Cuili Wang , Jun Liang , Pengfei Li , Kaida Xu , Hengtong Qiu

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Abstract

The common Chinese cuttlefish (Sepiella inermis) is an important cephalopod with nutritional and commercial value. Intensive inking stimulated by swilling seawater in transfer containers threatens the survival of cephalopods during transportation. However, the molecular basis for the inking behavior of S. inermis remains unclear. In the present study, transcriptome analysis was performed on ink sac and brain tissues from S. inermis under two different conditions, i.e. the control group (with individuals immersed in static seawater) and the experimental group (with individuals immersed in swilling seawater) to determine the global gene expression differences. The individuals from the experimental group ejected ink in response to the swilling of seawater. 330,699 unigenes were obtained from twelve transcriptome libraries via the Illumina Hiseq X platform, and the differentially expressed genes in the ink sac and brain tissues were identified respectively. Multiple upregulated genes in the ink sac were involved in cation transporter activity. Besides, an autocrine/paracrine factor wnt10b like and two important transcription factors (homeobox 1 and Hes-1-b-like) were also significantly upregulated in the ink sac. Moreover, a neuronal nitric oxide synthase (nNOS) was significantly downregulated in the brain. The findings from this study provide an important transcriptomic resource for discovering critical genes related to inking behavior of S. inermis, providing a basis for developing potential methods for protecting S. inermis from intensive inking.

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无足seppiella inmis墨迹囊和脑组织的转录组分析:发现与头足类墨迹相关的基因的资源

中国普通墨鱼(Sepiella inermis)是一种具有重要营养价值和商业价值的头足类动物。在运输过程中，由于运输容器中海水的流动而产生的强烈的墨迹会威胁到头足类动物的生存。然而，目前尚不清楚这种化合物的分子基础。本研究通过对照(浸泡在静态海水中)和实验组(浸泡在流动海水中)两种不同条件下的墨囊和脑组织转录组分析，确定了墨囊和脑组织的整体基因表达差异。实验组的个体对海水的冲刷做出反应，喷射出墨水。通过Illumina Hiseq X平台从12个转录组文库中获得330,699个unique，分别鉴定了墨囊和脑组织中的差异表达基因。墨囊中的多个上调基因参与了阳离子转运蛋白的活性。此外，自分泌/旁分泌因子wnt10b like和两个重要的转录因子(homeobox 1和hes -1-b like)也在墨囊中显著上调。此外，神经元一氧化氮合酶(nNOS)在大脑中显著下调。本研究结果为发现与水杨花粘接行为相关的关键基因提供了重要的转录组学资源，为开发保护水杨花免受密集粘接的潜在方法提供了基础。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.