Léanie Alloing-Séguier, Laurent Marivaux, Jean-François Barczi, Fabrice Lihoreau, Camille Martinand-Mari
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引用次数: 3
Abstract
Rodent enamel microstructure has been extensively investigated, primarily on the basis of 2D electronic microscopy data. The nature and dynamics of the ameloblasts (the enamel-secreting cells) have also been well studied. However, critical issues still remain surrounding exactly how the ameloblasts produce the astonishing microstructural complexity of enamel, and how this subtle architecture evolved through time. In this article, we used a new methodology based on confocal laser microscopy to reconstruct the enamel microstructure of rodent incisors in three dimensions (3D) with the ameloblasts in situ. We proposed interpretations regarding the possible relationships between the workings of the ameloblasts and the resulting enamel prisms, especially how the phenomenon of decussation is generated. Finally, we were able to represent the two main types of modern rodent incisor microstructures (uniserial and multiserial decussations), as a set of parameters that have been entered into the 3D enamel simulation software Simulenam to generate 3D models that can be digitally manipulated. Associating 2D data of incisor enamel microstructure of fossil rodents and Simulenam, it was then possible to better understand how the various decussation parameters evolved through time and gave rise to the two modern microstructure types from the same ancestral type (pauciserial). This study also confirmed that rodent and artiodactyl enamel do not share the same mechanism of decussation formation. Anat Rec, 302:1195-1209, 2019. © 2018 Wiley Periodicals, Inc.
啮齿动物门牙釉质棱柱状结构与成釉细胞层组织的关系。
啮齿类动物牙釉质微观结构已被广泛研究,主要基于二维电子显微镜数据。成釉细胞(分泌釉质的细胞)的性质和动力学也得到了很好的研究。然而,关于成釉细胞是如何产生惊人的珐琅质微观结构复杂性的,以及这种微妙的结构是如何随着时间的推移而进化的,仍然存在一些关键问题。本文采用激光共聚焦显微技术,利用成釉细胞原位重建啮齿动物门牙牙釉质三维显微结构。我们提出了关于成釉细胞的工作和由此产生的牙釉质棱镜之间的可能关系的解释,特别是如何产生讨论现象。最后,我们能够表示两种主要类型的现代啮齿动物切牙微结构(单序列和多序列讨论),作为一组参数,这些参数已经输入到3D牙釉质模拟软件Simulenam中,以生成可以数字操作的3D模型。将化石啮齿动物的门牙釉质微观结构的二维数据与Simulenam相结合,可以更好地理解各种讨论参数如何随着时间的推移而演变,并从同一祖先类型(pauciseri)中产生两种现代微观结构类型。本研究也证实了啮齿动物和偶蹄动物的牙釉质形成机制不同。中国生物医学工程学报,2019,32(2):1195- 1190。©2018 Wiley期刊公司
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