Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections

IF 4.8 Q2 NANOSCIENCE & NANOTECHNOLOGY

ACS Nanoscience Au Pub Date : 2022-04-04 DOI:10.1021/acsnanoscienceau.2c00001

Kyryl Zagorovsky, Maria Teresa Fernández-Argüelles, Diane Bona, Ashraf Mohamed Elshawadfy, Abdullah Muhammad Syed, Pranav Kadhiresan, Tony Mazzulli, Karen L. Maxwell and Warren C.W. Chan*,

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引用次数: 6

Abstract

Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10⁵ bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51–73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.

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诊断尿路感染的金纳米粒子智能手机平台

目前的尿路感染(UTI)诊断方法缓慢或提供有限的信息，导致在细菌病原体鉴定之前处方抗生素治疗。在这里，我们采用了金纳米颗粒比色法，并开发了一个用于UTI检测的智能手机平台。我们使用细菌特异性和通用探针在临床相关浓度为105个细菌/mL时平行鉴定了五种主要的UTI病原体。我们使用大肠杆菌、奇异变形杆菌和肺炎克雷伯菌感染患者的115个阳性和19个阴性样本验证了诊断技术。该试验在3小时内成功鉴定出感染病原体(特异性:98%，敏感性:51-73%)。我们的平台比培养更快，并且可以无线存储和传输结果，每次试验的成本为0.38美元。

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来源期刊

ACS Nanoscience Au 材料科学、纳米科学-

CiteScore

4.20

自引率

0.00%

发文量

期刊介绍： ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.