Photoresponsive Nanocarriers Based on Lithium Niobate Nanoparticles for Harmonic Imaging and On-Demand Release of Anticancer Chemotherapeutics

IF 4.8 Q2 NANOSCIENCE & NANOTECHNOLOGY
Adrian Gheata, Geoffrey Gaulier, Gabriel Campargue, Jérémy Vuilleumier, Simon Kaiser, Ivan Gautschi, Florian Riporto, Sandrine Beauquis, Davide Staedler, Dario Diviani, Luigi Bonacina and Sandrine Gerber-Lemaire*, 
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引用次数: 1

Abstract

Nanoparticle-based drug delivery systems have the potential for increasing the efficiency of chemotherapeutics by enhancing the drug accumulation at specific target sites, thereby reducing adverse side effects and mitigating patient acquired resistance. In particular, photo-responsive nanomaterials have attracted much interest due to their ability to release molecular cargos on demand upon light irradiation. In some settings, they can also provide complementary information by optical imaging on the (sub)cellular scale. We herein present a system based on lithium niobate harmonic nanoparticles (LNO HNPs) for the decoupled multi-harmonic cell imaging and near-infrared light-triggered delivery of an erlotinib derivative (ELA) for the treatment of epidermal growth factor receptor (EGFR)-overexpressing carcinomas. The ELA cargo was covalently conjugated to the surface of silica-coated LNO HNPs through a coumarinyl photo-cleavable linker, achieving a surface loading of the active molecule of 27 nmol/mg NPs. The resulting nanoconjugates (LNO-CM-ELA NPs) were successfully imaged upon pulsed laser excitation at 1250 nm in EGFR-overexpressing human prostate cancer cells DU145 by detecting the second harmonic emission at 625 nm, in the tissue transparency window. Tuning the laser at 790 nm resulted in the uncaging of the ELA cargo as a result of the second harmonic emission of the inorganic HNP core at 395 nm. This protocol induced a significant growth inhibition in DU145 cells, which was only observed upon specific irradiation at 790 nm, highlighting the promising capabilities of LNO-CM-ELA NPs for theranostic applications.

Abstract Image

基于铌酸锂纳米粒子的光响应纳米载体用于谐波成像和抗癌化疗药物的按需释放
基于纳米颗粒的药物输送系统有可能通过增强特定靶点的药物积累来提高化疗效率,从而减少不良副作用并减轻患者获得性耐药。特别是,光响应纳米材料由于其在光照射下释放分子货物的能力而引起了人们的极大兴趣。在某些情况下,它们还可以通过(亚)细胞尺度的光学成像提供补充信息。本文提出了一种基于铌酸锂谐波纳米粒子(LNO HNPs)的系统,用于解耦多谐波细胞成像和近红外光触发的厄洛替尼衍生物(ELA)递送,用于治疗表皮生长因子受体(EGFR)过表达的癌。ELA货物通过香豆素基光可切割连接剂共价结合到二氧化硅涂层的LNO HNPs表面,实现了活性分子27 nmol/mg NPs的表面负载。在1250 nm脉冲激光激发下,通过检测组织透明窗口625 nm的二次谐波发射,成功地对egfr过表达的人前列腺癌细胞DU145进行了纳米偶联物(LNO-CM-ELA NPs)的成像。将激光调谐到790 nm,由于无机HNP核心在395 nm处的二次谐波发射,导致ELA货物被释放。该方案在DU145细胞中诱导了显著的生长抑制,仅在790 nm的特定照射下观察到,突出了LNO-CM-ELA NPs在治疗应用方面的潜力。
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来源期刊
ACS Nanoscience Au
ACS Nanoscience Au 材料科学、纳米科学-
CiteScore
4.20
自引率
0.00%
发文量
0
期刊介绍: ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.
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