[The key role of the regulatory 19S subunit in changes in the brain proteasome subproteome induced by the neuroprotector isatin].

Q3 Biochemistry, Genetics and Molecular Biology
O A Buneeva, A T Kopylov, A E Medvedev
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引用次数: 0

Abstract

Isatin (indole-2,3-dione) is an endogenous regulator exhibiting various effects mediated by numerous isatin-binding proteins localized in different compartments of cells of the brain and peripheral tissues. It attenuates manifestations of experimental parkinsonism induced by administration of the MPTP neurotoxin and reduces the movement disorders characteristic of this disease. The molecular mechanisms of the neuroprotective action of isatin include its direct interaction with proteasomes, intracellular supramolecular complexes responsible for the targeted elimination of proteins. Incubation of fractions of 26S and 20S rabbit brain proteasomes, containing the whole spectrum of proteasomal subunits, as well as a number of proteasome-associated proteins, with isatin (100 μM) had a significant impact on the profile of released proteins. In the case of 26S proteasomes containing, in addition to the core part (20S proteasome), 19S regulatory subparticles, incubation with isatin resulted in a more than threefold increase in the number of dissociated proteins. In the case of 20S proteasomes (containing only the 20S core particle), incubation with isatin resulted in a significant decrease in the number of dissociated proteins compared to the control. Our results indicate an important role of the regulatory 19S subunit components in the formation of the proteasome subproteome and the sensitivity of these supramolecular complexes to isatin.

[调节19S亚基在神经保护剂isatin诱导的脑蛋白酶体亚蛋白质组变化中的关键作用]。
Isatin(吲哚-2,3-二酮)是一种内源性调节剂,由位于大脑和外周组织不同细胞区室的许多Isatin结合蛋白介导,具有多种作用。它减轻了MPTP神经毒素引起的实验性帕金森病的表现,并减少了这种疾病特有的运动障碍。isatin的神经保护作用的分子机制包括它与蛋白酶体的直接相互作用,蛋白酶体是细胞内的超分子复合物,负责靶向消除蛋白质。含有全谱蛋白酶体亚基以及多种蛋白酶体相关蛋白的26S和20S兔脑蛋白酶体与isatin (100 μM)孵育对释放的蛋白谱有显著影响。在含有26S蛋白酶体的情况下,除了核心部分(20S蛋白酶体)外,还含有19S调节亚颗粒,与isatin孵育导致解离蛋白数量增加三倍以上。在20S蛋白酶体(仅含有20S核心颗粒)的情况下,与对照相比,isatin孵育导致解离蛋白数量显著减少。我们的研究结果表明,调控的19S亚基组分在蛋白酶体亚蛋白质组的形成和这些超分子复合物对isatin的敏感性中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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