MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma.

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2022-12-01 DOI:10.1080/21691401.2022.2092123

Rawan Alswailem, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Bahauddeen M Alrfaei, Mohammad Badran, Qamraa Hamad Alqahtani, Hosam Gharib Abdelhady, Ibrahim Alsarra

{"title":"MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma.","authors":"Rawan Alswailem, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Bahauddeen M Alrfaei, Mohammad Badran, Qamraa Hamad Alqahtani, Hosam Gharib Abdelhady, Ibrahim Alsarra","doi":"10.1080/21691401.2022.2092123","DOIUrl":null,"url":null,"abstract":"Recent evidence has implicated microRNA-219 (miR-219) in regulation of gene contributed in glioblastoma (GBM) pathogenesis. This study aimed to prepare miR-219 in chitosan (CS) nanoparticles (NPs), characterize and investigate their efficacy on human GBM cell line (U87 MG). NPs were prepared using ionic gelation method. The influence of process parameters on physicochemical characteristics of NPs was investigated. Apoptotic effect of miR-219 was examined on U87 MG cells. Formulated NPs showed particle size of 109 ± 2.18 nm, with poly dispersity index equal to 0.2 ± 0.05, and zeta potential of +20.5 ± 0.7 mV. Entrapment efficiency of miR-219 in loaded NP has reached 95%. The in vitro release study demonstrated sustained release pattern of miR-219 from CS-NPs. Gel retardation assay has confirmed the integrity of miR-219 after production process. The fabricated NPs reduced the survival of U87 MG cells to 78% after 24 h of post-transfection, and into 67.5% after 48 h. However, fibroblasts were not affected by the NPs, revealing their specificity for GBM cells. Given the tumour suppressing function of miR-219, and advantage of CS-NPs for gene delivery to the central nervous system, the presented NPs have a great potential for treatment of GBM.","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":" ","pages":"198-207"},"PeriodicalIF":4.5000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial Cells, Nanomedicine, and Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/21691401.2022.2092123","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 7

Abstract

Recent evidence has implicated microRNA-219 (miR-219) in regulation of gene contributed in glioblastoma (GBM) pathogenesis. This study aimed to prepare miR-219 in chitosan (CS) nanoparticles (NPs), characterize and investigate their efficacy on human GBM cell line (U87 MG). NPs were prepared using ionic gelation method. The influence of process parameters on physicochemical characteristics of NPs was investigated. Apoptotic effect of miR-219 was examined on U87 MG cells. Formulated NPs showed particle size of 109 ± 2.18 nm, with poly dispersity index equal to 0.2 ± 0.05, and zeta potential of +20.5 ± 0.7 mV. Entrapment efficiency of miR-219 in loaded NP has reached 95%. The in vitro release study demonstrated sustained release pattern of miR-219 from CS-NPs. Gel retardation assay has confirmed the integrity of miR-219 after production process. The fabricated NPs reduced the survival of U87 MG cells to 78% after 24 h of post-transfection, and into 67.5% after 48 h. However, fibroblasts were not affected by the NPs, revealing their specificity for GBM cells. Given the tumour suppressing function of miR-219, and advantage of CS-NPs for gene delivery to the central nervous system, the presented NPs have a great potential for treatment of GBM.

查看原文本刊更多论文

载MicroRNA-219壳聚糖纳米颗粒治疗胶质母细胞瘤。

最近的证据表明，microRNA-219 (miR-219)参与了胶质母细胞瘤(GBM)发病机制的基因调控。本研究旨在制备壳聚糖(CS)纳米颗粒(NPs)中的miR-219，并对其对人GBM细胞系(U87 MG)的作用进行表征和研究。采用离子凝胶法制备NPs。研究了工艺参数对NPs理化特性的影响。检测miR-219对U87 MG细胞的凋亡作用。NPs的粒径为109±2.18 nm，聚分散指数为0.2±0.05,zeta电位为+20.5±0.7 mV。miR-219在负载NP中的包封效率达到95%。体外释放研究证实了CS-NPs中miR-219的持续释放模式。凝胶阻滞实验证实了miR-219在生产过程后的完整性。制备的NPs使U87 MG细胞转染24 h后存活率降至78%，转染48 h后存活率降至67.5%。然而，成纤维细胞不受NPs的影响，揭示了它们对GBM细胞的特异性。鉴于miR-219的肿瘤抑制功能，以及CS-NPs在向中枢神经系统传递基因方面的优势，所提出的NPs在治疗GBM方面具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.