Therapeutic effects of sulforaphane in ulcerative colitis: effect on antioxidant activity, mitochondrial biogenesis and DNA polymerization.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Redox Report Pub Date : 2022-12-01 DOI:10.1080/13510002.2022.2092378

Abdullah Alattar, Reem Alshaman, Mohammed M H Al-Gayyar

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引用次数: 17

Abstract

Objectives: Ulcerative colitis (UC), an inflammatory bowel disease, affects mucosal lining of colon leading to inflammation and ulcers. Sulforaphane is a natural compound obtained from cruciferous vegetables. We aimed to investigate potential therapeutic effects of sulforaphane in experimentally induced UC in rats through affection antioxidant activity, mitochondrial biogenesis and DNA polymerization.

Methods: UC was induced in rats via an intracolonic single administration of 2 ml of 4% acetic acid. UC rats were treated with 15 mg/kg sulforaphane. Samples of colon were used to investigate gene expression and protein levels of peroxisome proliferator-activated receptor-gamma coactivator (PGC-1), mitochondrial transcription factor A (TFAM), mammalian target of rapamycin (mTOR), cyclin D1, nuclear factor erythroid 2-related factor-2 (Nrf2), heme Oxygenase-1 (HO-1) and proliferating cell nuclear antigen (PCNA).

Results: UC showed dark distorted Goblet cell nucleus with disarranged mucus granules and no distinct brush border with atypical microvilli. All morphological changes were improved by treating with sulforaphane. Finally, treatment with sulforaphane significantly increased expression of PGC-1, TFAM, Nrf2 and HO-1 associated with reduction in expression of mTOR, cyclin D1 and PCNA.

Conclusion: Sulforaphane could cure UC in rats. The protective activity can be explained by enhancing antioxidant activity, elevating mitochondrial biogenesis and inhibiting DNA polymerization.

Abstract Image

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萝卜硫素对溃疡性结肠炎的治疗作用:对抗氧化活性、线粒体生物发生和DNA聚合的影响。

目的:溃疡性结肠炎(UC)是一种炎症性肠病，影响结肠粘膜，导致炎症和溃疡。萝卜硫素是从十字花科蔬菜中提取的天然化合物。本研究旨在探讨萝卜硫素对实验性UC大鼠抗氧化活性、线粒体生物发生和DNA聚合的影响。方法:大鼠结肠内单次给药2ml 4%乙酸诱导UC。UC大鼠给予15 mg/kg萝卜硫素处理。利用结肠样品检测过氧化物酶体增殖物激活受体- γ辅助激活因子(PGC-1)、线粒体转录因子A (TFAM)、哺乳动物雷帕霉素靶蛋白(mTOR)、细胞周期蛋白D1、核因子红系2相关因子-2 (Nrf2)、血红素加氧酶-1 (HO-1)和增殖细胞核抗原(PCNA)的基因表达和蛋白水平。结果:UC呈杯状细胞核黑色变形，黏液颗粒排列紊乱，无明显刷状边界，微绒毛不典型。萝卜硫素处理改善了所有形态学变化。最后，萝卜硫素处理显著增加PGC-1、TFAM、Nrf2和HO-1的表达，并降低mTOR、cyclin D1和PCNA的表达。结论:萝卜硫素可治疗大鼠UC。其保护作用可以通过增强抗氧化活性、促进线粒体生物发生和抑制DNA聚合来解释。

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来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.