Aspergillosis by cryptic Aspergillus species: A case series and review of the literature

IF 1.5 4区 生物学 Q4 MYCOLOGY
Mariana Fernandez-Pittol , Izaskun Alejo-Cancho , Elisa Rubio-García , Celia Cardozo , Pedro Puerta-Alcalde , Estela Moreno-García , Nicole Garcia-Pouton , Miriam Garrido , Miriam Villanueva , Ana Alastruey-Izquierdo , Cristina Pitart , Carolina Garcia-Vidal , Francesc Marco
{"title":"Aspergillosis by cryptic Aspergillus species: A case series and review of the literature","authors":"Mariana Fernandez-Pittol ,&nbsp;Izaskun Alejo-Cancho ,&nbsp;Elisa Rubio-García ,&nbsp;Celia Cardozo ,&nbsp;Pedro Puerta-Alcalde ,&nbsp;Estela Moreno-García ,&nbsp;Nicole Garcia-Pouton ,&nbsp;Miriam Garrido ,&nbsp;Miriam Villanueva ,&nbsp;Ana Alastruey-Izquierdo ,&nbsp;Cristina Pitart ,&nbsp;Carolina Garcia-Vidal ,&nbsp;Francesc Marco","doi":"10.1016/j.riam.2022.04.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The cryptic <em>Aspegillus</em> species are rare, these microorganisms are usually more resistant to common antifungal therapies. Therefore, a correct identification is important when evaluating the impact of such species in aspergillosis.</p></div><div><h3>Aims</h3><p>We aimed to describe the frequency, clinical and microbiological characteristics, and the outcomes of those cases of aspergillosis caused by cryptic species in a tertiary hospital.</p></div><div><h3>Methods</h3><p>We retrospectively identified all microbiologically documented cases of aspergillosis between January 2013 and December 2018. Definitive species identification of clinically significant isolates was achieved via sequencing methods. The polymerase chain reaction (PCR) products were sequenced, and the results obtained were compared to sequences deposited in GenBank. Antifungal susceptibility testing was performed using the Sensititre® YeastOne® panel.</p></div><div><h3>Results</h3><p>A total of 679 <span><em>Aspergillus</em></span> isolates were recovered from 489 patients, of which 109 were clinically relevant. Ten (9.2%) isolates were identified as cryptic species: <em>Aspergillus arcoverdensis</em> (2), <em>Aspergillus lentulus</em> (2), <em>Aspergillus ellipticus</em> (2), <em>Aspergillus alliaceus</em> (1), <em>Aspergillus nomius</em> (1), <em>Aspergillus tubingensis</em> (1) and <em>Aspergillus montevidensis</em> (1). Most patients already suffered some type of immunosuppression. Half of these patients had required intensive care before the infection showed up, and most of them had a pulmonary infection. Mortality at the 100-day follow-up was 40%. Antifungal susceptibility testing was performed on three of the isolates (<em>A. arcoverdensis</em>, <em>A. tubingensis</em> and <em>A. nomius</em><span>), which showed high minimum inhibitory concentrations (MIC) for azoles and amphotericin B.</span></p></div><div><h3>Conclusions</h3><p>The frequency of cryptic species in our centre was 9.2%. Most patients had some degree of immunosuppression, and the mortality rate was 40%.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1130140622000195","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 4

Abstract

Background

The cryptic Aspegillus species are rare, these microorganisms are usually more resistant to common antifungal therapies. Therefore, a correct identification is important when evaluating the impact of such species in aspergillosis.

Aims

We aimed to describe the frequency, clinical and microbiological characteristics, and the outcomes of those cases of aspergillosis caused by cryptic species in a tertiary hospital.

Methods

We retrospectively identified all microbiologically documented cases of aspergillosis between January 2013 and December 2018. Definitive species identification of clinically significant isolates was achieved via sequencing methods. The polymerase chain reaction (PCR) products were sequenced, and the results obtained were compared to sequences deposited in GenBank. Antifungal susceptibility testing was performed using the Sensititre® YeastOne® panel.

Results

A total of 679 Aspergillus isolates were recovered from 489 patients, of which 109 were clinically relevant. Ten (9.2%) isolates were identified as cryptic species: Aspergillus arcoverdensis (2), Aspergillus lentulus (2), Aspergillus ellipticus (2), Aspergillus alliaceus (1), Aspergillus nomius (1), Aspergillus tubingensis (1) and Aspergillus montevidensis (1). Most patients already suffered some type of immunosuppression. Half of these patients had required intensive care before the infection showed up, and most of them had a pulmonary infection. Mortality at the 100-day follow-up was 40%. Antifungal susceptibility testing was performed on three of the isolates (A. arcoverdensis, A. tubingensis and A. nomius), which showed high minimum inhibitory concentrations (MIC) for azoles and amphotericin B.

Conclusions

The frequency of cryptic species in our centre was 9.2%. Most patients had some degree of immunosuppression, and the mortality rate was 40%.

由隐曲霉引起的曲霉病:一个病例系列和文献综述
背景:隐曲霉种类是罕见的,这些微生物通常对常见的抗真菌治疗更耐药。因此,在评估这些菌种对曲霉病的影响时,正确的鉴定是很重要的。目的了解某三级医院隐匿菌种引起的曲霉病的发病频率、临床和微生物学特征及转归。方法回顾性分析2013年1月至2018年12月期间所有微生物学记录的曲霉病病例。通过测序方法确定了具有临床意义的分离株的最终物种鉴定。对聚合酶链反应(PCR)产物进行测序,并将测序结果与存放在GenBank中的序列进行比较。使用Sensititre®YeastOne®面板进行抗真菌药敏试验。结果从489例患者中共检出曲霉679株,其中与临床相关的109株。10株(9.2%)被鉴定为隐种:arcoverdensis Aspergillus(2)、lentulus Aspergillus(2)、ellipticus Aspergillus(2)、alliaceus Aspergillus(1)、nomius Aspergillus(1)、tubingaspergillus(1)和montevidensis Aspergillus(1)。大多数患者已经出现某种类型的免疫抑制。这些患者中有一半在感染出现之前需要重症监护,其中大多数患有肺部感染。100天随访死亡率为40%。对3株菌株(arcoverdensis、tubingensis和nomius)进行了药敏试验,结果表明3株菌株对唑类和两性霉素b具有较高的最低抑菌浓度(MIC)。多数患者存在不同程度的免疫抑制,死亡率为40%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
81 days
期刊介绍: Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信