Follistatin Protein Enhances Satellite Cell Counts in Reinnervated Muscle.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2022-06-21 eCollection Date: 2022-01-01 DOI:10.1055/s-0042-1748535
Mark A Feger, Jonathan Isaacs, Satya Mallu, Dorne Yager, Mary Shall, Gaurangkumar Patel, Omar Protzuk, Akhil S Bokkisam
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引用次数: 0

Abstract

Background Muscle recovery following peripheral nerve repair is sup-optimal. Follistatin (FST), a potent muscle stimulant, enhances muscle size and satellite cell counts following reinnervation when administered as recombinant FST DNA via viral vectors. Local administration of recombinant FST protein, if effective, would be more clinically translatable but has yet to be investigated following muscle reinnervation. Objective  The aim of this study is to assess the effect of direct delivery of recombinant FST protein on muscle recovery following muscle reinnervation. Materials and Methods  In total, 72 Sprague-Dawley rats underwent temporary (3 or 6 months) denervation or sham denervation. After reinnervation, rats received FST protein (isoform FS-288) or sham treatment via a subcutaneous osmotic pump delivery system. Outcome measures included muscle force, muscle histomorphology, and FST protein quantification. Results  Follistatin treatment resulted in smaller muscles after 3 months denervation ( p  = 0.019) and reduced force after 3 months sham denervation ( p  < 0.001). Conversely, after 6 months of denervation, FST treatment trended toward increased force output ( p  = 0.066). Follistatin increased satellite cell counts after denervation ( p  < 0.001) but reduced satellite cell counts after sham denervation ( p  = 0.037). Conclusion  Follistatin had mixed effects on muscle weight and force. Direct FST protein delivery enhanced satellite cell counts following reinnervation. The positive effect on the satellite cell population is intriguing and warrants further investigation.

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Follistatin 蛋白能增强再神经肌肉中卫星细胞的数量。
背景外周神经修复后的肌肉恢复效果并不理想。Follistatin (FST) 是一种强效的肌肉刺激剂,通过病毒载体以重组 FST DNA 的形式给药时,能增强神经再支配后的肌肉大小和卫星细胞数量。重组 FST 蛋白的局部给药如果有效,将更适用于临床,但尚未在肌肉神经再支配后进行研究。本研究旨在评估直接输送重组 FST 蛋白对肌肉神经支配后肌肉恢复的影响。材料和方法 共有 72 只 Sprague-Dawley 大鼠接受了暂时性(3 或 6 个月)去神经支配或假性去神经支配。神经再支配后,大鼠通过皮下渗透泵给药系统接受 FST 蛋白(异构体 FS-288)或假治疗。结果测量包括肌力、肌肉组织形态学和 FST 蛋白定量。结果 福利他汀治疗 3 个月后肌肉变小(P = 0.019),假性去神经治疗 3 个月后肌肉力量减弱(P = 0.066)。去神经支配后,福利他汀增加了卫星细胞数量(P = 0.037)。结论 福利他汀对肌肉重量和力量的影响不一。直接输送 FST 蛋白质可提高神经再支配后的卫星细胞数量。这种对卫星细胞数量的积极影响令人好奇,值得进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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