A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior.

IF 1.1 Q4 CLINICAL NEUROLOGY
Journal of Brachial Plexus and Peripheral Nerve Injury Pub Date : 2022-06-21 eCollection Date: 2022-01-01 DOI:10.1055/s-0042-1747959
Jonathan A Sorkin, Ziv Rechany, Mara Almog, Nina Dietzmeyer, Yuval Shapira, Kirsten Haastert-Talini, Shimon Rochkind
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Abstract

Background  The rabbit sciatic nerve injury model may represent a valuable alternative for critical gap distance seen in humans but often leads to automutilation. In this study, we modified the complete sciatic nerve injury model for avoiding autophagy. Materials and Methods  In 20 adult female New Zealand White rabbits, instead of transecting the complete sciatic nerve, we unilaterally transected the tibial portion and preserved the peroneal portion. Thereby loss of sensation in the dorsal aspect of the paw was avoided. The tibial portion was repaired in a reversed autograft approach in a length of 2.6 cm. In an alternative repair approach, a gap of 2.6 cm in length was repaired with a chitosan-based nerve guide. Results  During the 6-month follow-up period, there were no incidents of autotomy. Nerve regeneration of the tibial portion of the sciatic nerve was evaluated histologically and morphometrically. A clear difference between the distal segments of the healthy contralateral and the repaired tibial portion of the sciatic nerve was detectable, validating the model. Conclusion  By transecting the isolated tibial portion of the rabbit sciatic nerve and leaving the peroneal portion intact, it was possible to eliminate automutilation behavior.

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避免自动化行为的兔周围神经重建模型研究。
兔坐骨神经损伤模型可能是人类临界间隙距离的一个有价值的替代方法,但往往导致自动化。在本研究中,我们改良了完整的坐骨神经损伤模型以避免自噬。材料与方法20只成年雌性新西兰大白兔,不切除完整坐骨神经,单侧切除胫骨部分,保留腓骨部分。这样就避免了爪背的感觉丧失。胫骨部分采用反向自体移植入路修复,长度为2.6 cm。在另一种修复方法中,用壳聚糖为基础的神经引导物修复了2.6厘米长的间隙。结果随访6个月,未发生自切手术。对坐骨神经胫骨部分的神经再生进行组织学和形态计量学评价。健康对侧和修复后的坐骨神经胫骨部分的远端节段明显不同,验证了该模型。结论横断离体兔坐骨神经胫骨部分,保留腓神经部分完整,可以消除自动性行为。
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来源期刊
CiteScore
1.70
自引率
14.30%
发文量
6
审稿时长
12 weeks
期刊介绍: JBPPNI is an open access, peer-reviewed online journal that will encompass all aspects of basic and clinical research findings, in the area of brachial plexus and peripheral nerve injury. Injury in this context refers to congenital, inflammatory, traumatic, degenerative and neoplastic processes, including neurofibromatosis. Papers on diagnostic and imaging aspects of the peripheral nervous system are welcomed as well. The peripheral nervous system is unique in its complexity and scope of influence. There are areas of interest in the anatomy, physiology, metabolism, phylogeny, and limb growth tropism of peripheral nerves.
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