Enhanced olfactory memory detection in trap-design Y-mazes allows the study of imperceptible memory traces in Drosophila.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Radhika Mohandasan, Manikrao Thakare, Suhas Sunke, Fathima Mukthar Iqbal, Madhav Sridharan, Gaurav Das
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引用次数: 2

Abstract

The neural basis of behavior is identified by systematically manipulating the activity of specific neurons and screening for loss or gain of phenotype. Therefore, robust, high-scoring behavioral assays are necessary for determining the neural circuits of novel behaviors. We report a simple Y-maze design for Drosophila olfactory learning and memory assay. Memory scores in our Y-mazes are considerably better and longer-lasting than scores obtained with commonly used T-mazes. Our results suggest that trapping flies to an odor choice in a Y-maze could improve scores. We postulated that the improved scores could reveal previously undetectable memory traces, enabling the study of underlying neural mechanisms. Indeed, we identified unreported protein synthesis-dependent long-term memories (LTMs), reinforced by ingestion of (1) an aversive compound and (2) a sweet but nonnutritious sugar, both 24 h after training. We also used Y-mazes to probe how using a greater reward may change memory dynamics. Our findings predict that a greater sugar reward may extend existing memory traces or reinforce additional novel ones.

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陷阱设计Y迷宫中增强的嗅觉记忆检测可以研究果蝇的难以察觉的记忆痕迹。
行为的神经基础是通过系统地操纵特定神经元的活动和筛选表型的损失或获得来识别的。因此,稳健、高评分的行为分析对于确定新行为的神经回路是必要的。我们报道了一种用于果蝇嗅觉学习和记忆测定的简单Y迷宫设计。我们的Y迷宫的记忆得分比常用的T迷宫的得分要好得多,持续时间也更长。我们的研究结果表明,将苍蝇困在Y型迷宫中的气味选择中可以提高分数。我们假设,分数的提高可以揭示以前无法检测到的记忆痕迹,从而能够研究潜在的神经机制。事实上,我们发现了未报告的蛋白质合成依赖性长期记忆(LTM),在训练后24小时摄入(1)一种令人厌恶的化合物和(2)一种甜但无营养的糖会增强LTM。我们还使用Y迷宫来探索使用更大的奖励如何改变记忆动力学。我们的研究结果预测,更大的糖奖励可能会延长现有的记忆痕迹,或强化额外的新记忆痕迹。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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