Guo-Zhu Su , Shang-Yi Wang , Xiu-Ying Yang , Zora Dajić Stevanović , Na Li , Nikola Tanić , Nebojsa Arsenijević , Shi-Shan Yu , Yong Li
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引用次数: 1
Abstract
Five undescribed dihydroflavonoid glycoside derivatives, namely albvisosides A‒E, together with two known compounds were isolated from the roots and stem leaves of Viscum album L. var. album. (European mistletoe). Their structures were determined by HRESIMS, 1D and 2D NMR, and ECD analysis. Albvisoside B exhibits significant inhibitory effect on hepatic lipid accumulation in HepG2 cells at very low concentrations (EC50: 0.7 nM). Using proteome integral solubility alteration assay, the direct targets or downstream effectors of albvisoside B were elucidated. As a result, 97 proteins were identified based on ligand-induced alterations in the protein thermal stability. Bioinformatics analysis indicated that albvisoside B primarily ameliorated oleic acid-induced lipid accumulation by regulating the selenoamino acids metabolism signaling pathway. RPL3, ADAM17, and RPL14 were likely to be involved in mediating the lipid-lowering effect of albvisoside B.
从Viscum album L. var. album的根和茎叶中分离得到5个未描述的二氢黄酮苷衍生物,即白visvisosides A-E和2个已知的化合物。(欧洲槲寄生)。通过hresms、1D、2D NMR和ECD分析确定了它们的结构。在极低浓度(EC50: 0.7 nM)下,Albvisoside B对HepG2细胞的肝脏脂质积累有显著的抑制作用。利用蛋白质组积分溶解度改变实验,阐明了白维苷B的直接靶点或下游效应物。结果,基于配体诱导的蛋白质热稳定性改变,鉴定了97个蛋白质。生物信息学分析表明白维苷B主要通过调节硒氨基酸代谢信号通路改善油酸诱导的脂质积累。RPL3、ADAM17和RPL14可能参与了白藜芦醇B的降脂作用。
期刊介绍:
Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.