Cell-free methylation of RASSF1 and CDKN2AIP genes in the diagnosis of hepatocellular carcinoma associated with hepatitis B virus cirrhosis.

IF 1.2 Q4 GASTROENTEROLOGY & HEPATOLOGY

Hepatology Forum Pub Date : 2022-09-23 eCollection Date: 2022-09-01 DOI:10.14744/hf.2022.2022.0021

Pelin Telli, Nazli Begum Ozturk, Mehmet Tolgahan Hakan, Bilger Cavus, Asli Cifcibasi Ormeci, Aysun Yakut, Volkan Senkal, Ziya Imanov, Arzu Poyanli, Emine Goknur Isik, Kadir Demir, Fatih Besisik, Sabahattin Kaymakoglu, Ilhan Yaylim, Filiz Akyuz

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引用次数: 1

Abstract

Background and aim: Chronic hepatitis B virus (HBV) infection is a major cause of hepatocellular carcinoma (HCC). Circulating cell-free DNA (cfDNA) methylation of tumor suppressor genes are emerging potential biomarkers in HCC. We aimed to evaluate the cfDNA methylation status of RASSF1 and CDKN2AIP genes in patients with liver cirrhosis (LC) with or without HCC caused by HBV.

Materials and methods: A total of 47 patients with HBV cirrhosis were included in the study. Patients were divided into two groups: HCC and LC (HCC+LC, n=22) and HBV cirrhosis only (LC, n=25). cfDNA was isolated from the plasma samples of the patients. Methylation analysis was performed for RASSF1 and CDKN2AIP genes.

Results: Mean methylation percentage of CDKN2AIP gene was 0.001±0.004% in the HCC+LC group and 0.008±0.004 % in the LC only group. The mean methylation percentage of RASSF1 gene was 5.1±16.1% in the HCC+LC group and 9.7±25.9% in the LC only group. The methylation rate of CDKN2AIP was significantly lower in the HCC+LC group (p=0.027). A positive correlation was found with the absence of cfDNA methylation of CDKN2AIP gene in the presence of HCC (R=0.667, p=0.018).

Conclusion: cfDNA methylation of CDKN2AIP and RASSF1 genes may provide important diagnostic information regarding the development of HCC in the setting of HBV cirrhosis.

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RASSF1和CDKN2AIP基因的细胞游离甲基化在乙型肝炎病毒肝硬化相关肝细胞癌诊断中的作用

背景与目的:慢性乙型肝炎病毒(HBV)感染是导致肝细胞癌(HCC)的主要原因。肿瘤抑制基因的循环游离DNA (cfDNA)甲基化是HCC中新兴的潜在生物标志物。我们旨在评估伴有或不伴有HBV引起的HCC的肝硬化(LC)患者的RASSF1和CDKN2AIP基因的cfDNA甲基化状态。材料与方法:共纳入47例乙型肝炎肝硬化患者。患者分为两组:HCC和LC (HCC+LC, n=22)和HBV肝硬化(LC, n=25)。从患者血浆样本中分离cfDNA。对RASSF1和CDKN2AIP基因进行甲基化分析。结果:HCC+LC组CDKN2AIP基因平均甲基化率为0.001±0.004%，LC组为0.008±0.004%。HCC+LC组RASSF1基因的平均甲基化率为5.1±16.1%，LC组为9.7±25.9%。CDKN2AIP甲基化率在HCC+LC组显著降低(p=0.027)。CDKN2AIP基因cfDNA甲基化缺失与HCC存在正相关(R=0.667, p=0.018)。结论:CDKN2AIP和RASSF1基因的cfDNA甲基化可能为HBV肝硬化患者HCC的发展提供重要的诊断信息。

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来源期刊

Hepatology Forum

CiteScore

1.90

自引率

12.50%

发文量