Optimization of differentiation protocols of dental tissues stem cells to pancreatic β-cells.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2022-09-20 DOI:10.1186/s12860-022-00441-6

Riham M Aly, Hadeer A Aglan, Ghada Nour Eldeen, Hanaa H Ahmed

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引用次数: 0

Abstract

Background: Despite the recent progress in the differentiation strategies of stem cells into pancreatic beta cell lineage, current protocols are not optimized for different cell types. The purpose of this study is to investigate and compare the ability of stem cells derived from dental pulp (DPSCs) and periodontal ligament (PDLSCs) as two anatomically different dental tissues to differentiate into pancreatic beta cells while assessing the most suitable protocol for each cell type.

Methods: DPSCs & PDLSCs were isolated and characterized morphologically and phenotypically and then differentiated into pancreatic beta cells using two protocols. Differentiated cells were assessed by qRT-PCR for the expression of pancreatic related markers Foxa-2, Sox-17, PDX-1, Ngn-3, INS and Gcg. Functional assessment of differentiation was performed by quantification of Insulin release via ELISA.

Results: Protocol 2 implementing Geltrex significantly enhanced the expression levels of all tested genes both in DPSCs & PDLSCs. Both DPSCs & PDLSCs illustrated improved response to increased glucose concentration in comparison to undifferentiated cells. Moreover, DPSCs demonstrated an advanced potency towards pancreatic lineage differentiation over PDLSCs under both protocols.

Conclusion: In conclusion, the current study reports the promising potential of dental derived stem cells in differentiating into pancreatic lineage through selection of the right protocol.

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牙组织干细胞向胰腺β细胞分化方案的优化。

背景:尽管干细胞分化为胰腺β细胞谱系的策略最近取得了进展，但目前的方案并未针对不同的细胞类型进行优化。本研究的目的是研究和比较牙髓(DPSCs)和牙周韧带(PDLSCs)干细胞作为两种解剖结构不同的牙齿组织分化为胰腺β细胞的能力，同时评估每种细胞类型的最合适方案。方法:分离DPSCs和PDLSCs，对其进行形态学和表型鉴定，并采用两种方法分化为胰腺β细胞。采用qRT-PCR检测分化细胞中胰腺相关标志物Foxa-2、Sox-17、PDX-1、Ngn-3、INS和Gcg的表达情况。通过酶联免疫吸附测定胰岛素释放量来评估分化功能。结果:使用Geltrex的方案2显著提高了所有测试基因在DPSCs和PDLSCs中的表达水平。与未分化细胞相比，DPSCs和PDLSCs对葡萄糖浓度升高的反应都有所改善。此外，在两种方案下，DPSCs都表现出比PDLSCs更强的胰腺谱系分化能力。结论:总之，目前的研究报告了通过选择正确的方案，牙源性干细胞分化为胰腺谱系的良好潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.