RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL.

Q4 Medicine

African Journal of Infectious Diseases Pub Date : 2022-08-17 eCollection Date: 2022-01-01 DOI:10.21010/Ajid.v16i2S.8

Belefquih Bouchra, Maher Wissal, Cheikh Amine, Hamdani Wail, Zaid Younes, Kabbaj Hakima, Seffar Meryem, Dakka Hanae, Hadami Khaoula, Allaoui Afaf, Benouda Amina

{"title":"RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL.","authors":"Belefquih Bouchra, Maher Wissal, Cheikh Amine, Hamdani Wail, Zaid Younes, Kabbaj Hakima, Seffar Meryem, Dakka Hanae, Hadami Khaoula, Allaoui Afaf, Benouda Amina","doi":"10.21010/Ajid.v16i2S.8","DOIUrl":null,"url":null,"abstract":"Background: The main challenge faced in the African countries was to implement efficient molecular diagnostic facilities and start Covid-19 diagnosis as fast as possible to handle the rapid and unpredictable rise of cases.Materials methods and results: We describe our experience in implementing a molecular biology unit at Sheikh Zaïd International University Hospital in Rabat, with a delay as short as one week, and starting real-time RT-PCR assay for the detection of SARS-Cov-2 infection, since the outbreak widened in Morocco in mid-March, 2020.Conclusion: The challenges encountered in the first period of Covid-19 pandemic are still present. This work aims to give an example of a rapid and adaptive response in order to maintain our diagnosis ability for Covid-19 and for other pathogens.","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"16 2 Suppl","pages":"72-77"},"PeriodicalIF":0.0000,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480888/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Journal of Infectious Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21010/Ajid.v16i2S.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Background: The main challenge faced in the African countries was to implement efficient molecular diagnostic facilities and start Covid-19 diagnosis as fast as possible to handle the rapid and unpredictable rise of cases.

Materials methods and results: We describe our experience in implementing a molecular biology unit at Sheikh Zaïd International University Hospital in Rabat, with a delay as short as one week, and starting real-time RT-PCR assay for the detection of SARS-Cov-2 infection, since the outbreak widened in Morocco in mid-March, 2020.

Conclusion: The challenges encountered in the first period of Covid-19 pandemic are still present. This work aims to give an example of a rapid and adaptive response in order to maintain our diagnosis ability for Covid-19 and for other pathogens.

Abstract Image

查看原文本刊更多论文

在摩洛哥一家医院快速实施实时反转录聚合酶链反应(real-time rt-pcr)检测sars-cov-2

背景:非洲国家面临的主要挑战是实施高效的分子诊断设施，并尽快启动Covid-19诊断，以应对病例的快速和不可预测的增长。材料、方法和结果:我们描述了自2020年3月中旬摩洛哥疫情扩大以来，我们在拉巴特谢赫Zaïd国际大学医院实施分子生物学单元(延迟时间短至一周)和启动实时RT-PCR检测检测SARS-Cov-2感染的经验。结论:新冠肺炎大流行初期面临的挑战依然存在。这项工作旨在提供一个快速和适应性反应的例子，以保持我们对Covid-19和其他病原体的诊断能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

African Journal of Infectious Diseases Medicine-Infectious Diseases

CiteScore

1.60

自引率

0.00%

发文量