Cytotoxic Activity and Phytochemical Analysis of Artemisia haussknechtii Boiss.

Iranian Journal of Pharmaceutical Research : IJPR Pub Date : 2022-04-30 eCollection Date: 2022-12-01 DOI:10.5812/ijpr-126917

Sajjad Nasseri, Mohammad-Reza Delnavazi, Farshad H Shirazi, Faraz Mojab

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Abstract

Cytotoxic activity of crude extract and fractions (petroleum ether, dichloromethane, and n-butanol) of Artemisia haussknechtii aerial parts was investigated by MTT assay. Dichloromethane fraction showed the highest cytotoxic effect on MCF-7 cell line (IC₅₀ = 297.17 ± 7.99 µg/mL). Phytochemical analysis of the most effective fraction was carried out using normal phase column chromatography (CC) to get eight sub-fractions (A-H). Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used for further purification. Four known compounds with cytotoxic effects on cancer cell lines were isolated from the most active fraction, including 5-Hydroxy-3',4',6,7-tetramethoxyflavone (eupatilin 7-methyl ether), 5-hydroxy 3,3',4',6,7-pentamethoxy-flavone (artemetin), 6-methoxy-7-hydroxycoumarin (scopoletin), and methyl caffeate. Structure elucidation of isolated compounds was done using spectroscopic techniques, including ESIMS and 1D-NMR (¹H and ¹³C). Cytotoxic activity of A. haussknechtii is probably due to coumarin and flavonoid compounds.

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黄花蒿的细胞毒活性及植物化学分析。

采用MTT法研究了黄花蒿地皮粗提物和石油醚、二氯甲烷、正丁醇组分的细胞毒活性。二氯甲烷组分对MCF-7细胞株的细胞毒作用最高(IC50 = 297.17±7.99µg/mL)。采用正相柱色谱法(CC)对最有效组分进行植物化学分析，得到8个亚组分(A-H)。采用薄层色谱(TLC)和高效液相色谱(HPLC)进行进一步纯化。从活性部位分离得到4个已知的对癌细胞具有细胞毒作用的化合物，包括5-羟基-3′，4′，6,7-四甲基甲氧基黄酮(euupatilin 7-甲基醚)，5-羟基3,3′，4′，6,7-五甲基甲氧基黄酮(蒿甲素)，6-甲氧基-7-羟基香豆素(东莨菪碱)和咖啡酸甲酯。利用ESIMS和1D-NMR (1H和13C)等波谱技术对分离化合物进行了结构分析。haussknechtii的细胞毒活性可能与香豆素和黄酮类化合物有关。

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Iranian Journal of Pharmaceutical Research : IJPR

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