Lipopolysaccharide-preconditioned allogeneic adipose-derived stem cells improve erectile function in a rat model of bilateral cavernous nerve injury.

IF 2.4 3区医学 Q2 ANDROLOGY

Basic and Clinical Andrology Pub Date : 2022-03-25 DOI:10.1186/s12610-022-00156-w

Zhenbin Zhang, Pan Nie, Wende Yang, Xiaolei Ma, Zehong Chen, Hongbo Wei

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引用次数: 3

Abstract

Background: Erectile dysfunction (ED) often occurs due to cavernous nerve injury (CNI) after colorectal surgery. Cell-based therapies have great potential for the treatment of CNI-related ED; however, it needs to be optimised. In this study, we explored the therapeutic effects of lipopolysaccharide-preconditioned allogeneic adipose-derived stem cells (L-ADSCs) on CNI-induced ED in rats.

Results: The results of this in vitro study revealed that low-dose lipopolysaccharide could increase the viability of ADSCs, inhibit caspase 3 activation induced by hydrogen peroxide and promote cell migration. Compared with the ADSC supernatant, the L-ADSC supernatant could better reduce fibrosis in the corpus cavernosum smooth muscle cells induced by transforming growth factor-beta 1 protein. In the in vivo study, it was compared to ADSCs therapy, where the L-ADSCs therapy indicated that could better improve erectile function by increasing smooth muscle content and alleviating penile fibrosis in rats 2 weeks after CNI. The outcome may be related to the increase in the hepatocyte growth factor content in the corpus cavernosum and myelin basic protein in the major pelvic ganglion.

Conclusions: L-ADSC treatment may be a promising approach for restoring erectile function after CNI.

Abstract Image

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脂多糖预处理异体脂肪干细胞改善双侧海绵状神经损伤大鼠模型的勃起功能。

背景:结直肠手术后海绵状神经损伤常导致勃起功能障碍(ED)。细胞疗法在cni相关ED的治疗中具有巨大的潜力;然而，它需要被优化。在本研究中，我们探讨了脂多糖预处理异体脂肪来源干细胞(L-ADSCs)对cni诱导的大鼠ED的治疗作用。结果:体外实验结果显示，低剂量脂多糖可提高ADSCs的活力，抑制过氧化氢诱导的caspase 3活化，促进细胞迁移。与ADSC上清相比，L-ADSC上清能更好地减轻转化生长因子- β 1蛋白诱导的海绵体平滑肌细胞纤维化。在体内研究中，将其与ADSCs治疗进行了比较，结果表明，L-ADSCs治疗可以通过增加CNI后2周大鼠的平滑肌含量和减轻阴茎纤维化来更好地改善勃起功能。结果可能与海绵体中肝细胞生长因子含量和盆腔神经节中髓鞘碱性蛋白含量的增加有关。结论:L-ADSC治疗可能是CNI后恢复勃起功能的一种有希望的方法。

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来源期刊

Basic and Clinical Andrology Medicine-Urology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Basic and Clinical Andrology is an open access journal in the domain of andrology covering all aspects of male reproductive and sexual health in both human and animal models. The journal aims to bring to light the various clinical advancements and research developments in andrology from the international community.