RAD6 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma by Regulating Histone Ubiquitination of CCNB1.

IF 4.3 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Biological Procedures Online Pub Date : 2022-03-23 DOI:10.1186/s12575-022-00165-z

Yu Deng, Yujiang Li, Tiantong Wu, Xuyuan Chen, Xiang Li, Kaican Cai, Xu Wu

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引用次数: 0

Abstract

Objective Esophageal carcinoma (ESCA) is deadly cancer worldwide with unknown etiology. This study aimed to investigate the impact and mechanism of RAD6 on the development of Esophageal squamous cell carcinoma (ESCC).Expressions of RAD6A and RAD6B in ESCA were investigated from TCGA dataset and their expressions in tissue sample of ESCA patients and cells were determined. Functional experiments were conducted to explore the impact of RAD6A and RAD6B on malignant characteristics of several kinds of ESCC cells. Animal experiment was established and injected with RAD6A and RAD6B shRNA to evaluate the effect on tumor growth.RAD6A and RAD6B were up-regulated in ESCC cells and tissues. Overexpressed RAD6A and RAD6B similarly increased ESCC cell proliferation, invasion and migration and silencing of RAD6 exerted opposite effects. Knockdown of RAD6A suppressed tumor growth and decreased the level of H2B, as data demonstrated positive correlation between RAD6A and CCNB1 in ESCC tissues.Collectively, this study elucidates that RAD6 is up-regulated in ESCC and promotes the progression of ESCC through up-regulation of CCNB1 to enhance H2B ubiquitination. These evidence provide a novel insight into the pathogenesis of ESCC and might contribute to the development of targeted therapy.

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RAD6通过调节CCNB1的组蛋白泛素化积极影响食管鳞状细胞癌的发生。

目的食管癌是世界范围内病因不明的致命肿瘤。本研究旨在探讨RAD6在食管鳞状细胞癌(ESCC)发生发展中的作用及其机制。在TCGA数据集中研究了RAD6A和RAD6B在ESCA中的表达，并测定了它们在ESCA患者组织样本和细胞中的表达。通过功能实验探讨RAD6A和RAD6B对几种ESCC细胞恶性特征的影响。建立动物实验，注射RAD6A和RAD6B shRNA，评价其对肿瘤生长的影响。RAD6A和RAD6B在ESCC细胞和组织中表达上调。RAD6A和RAD6B的过表达同样增加了ESCC细胞的增殖、侵袭和迁移，而RAD6的沉默则起到相反的作用。敲低RAD6A抑制肿瘤生长，降低H2B水平，数据显示在ESCC组织中RAD6A与CCNB1呈正相关。综上所述，本研究阐明了RAD6在ESCC中上调，并通过上调CCNB1增强H2B泛素化，促进ESCC的进展。这些证据为ESCC的发病机制提供了新的见解，并可能有助于靶向治疗的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biological Procedures Online 生物-生化研究方法

CiteScore

10.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： iological Procedures Online publishes articles that improve access to techniques and methods in the medical and biological sciences. We are also interested in short but important research discoveries, such as new animal disease models. Topics of interest include, but are not limited to: Reports of new research techniques and applications of existing techniques Technical analyses of research techniques and published reports Validity analyses of research methods and approaches to judging the validity of research reports Application of common research methods Reviews of existing techniques Novel/important product information Biological Procedures Online places emphasis on multidisciplinary approaches that integrate methodologies from medicine, biology, chemistry, imaging, engineering, bioinformatics, computer science, and systems analysis.