Primary, secondary and compensated male biochemical hypogonadism in people living with HIV (PLWH): relevance of sex hormone-binding globulin (SHBG) measurement and comparison between liquid chromatography-tandem mass spectrometry (LC-MS/MS) and chemiluminescent immunoassay for sex steroids assay.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2022-12-01 DOI:10.1080/13685538.2022.2039116

Sara De Vincentis, Maria Chiara Decaroli, Flaminia Fanelli, Chiara Diazzi, Marco Mezzullo, Giulia Tartaro, Simonetta Tagliavini, Maria Cristina De Santis, Laura Roli, Jovana Milic, Tommaso Trenti, Uberto Pagotto, Giovanni Guaraldi, Vincenzo Rochira

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引用次数: 5

Abstract

Background: Data about classification of hypogonadism and estrogen deficiency in male people living with HIV (PLWH) are scanty.

Aim: To investigate the prevalence and characterization of biochemical hypogonadism and relative estrogen deficiency in male PLWH aged < 50 comparing liquid chromatography-tandem mass spectrometry (LC-MS/MS) with chemiluminescent immunoassay (CI), and combining gonadotropin, sex hormone-binding globulin (SHBG) and serum estradiol (E2) measurements.

Methods: Prospective, cross-sectional, observational study. Serum total testosterone (TT), E2, gonadotropins, SHBG were measured by CI. TT and E2 were also assessed by LC-MS/MS. Free testosterone (cFT) was calculated by Vermeulen equation.

Results: A total of 316 PLWH (45.3 ± 5.3 years) were enrolled. TT and cFT by LC-MS/MS were lower compared to CI (p < 0.0001). The prevalence of biochemical hypogonadism was higher with LC-MS/MS than CI, both for TT (5.1% vs 3.2%, p < 0.0001) or cFT (9.5% vs 7%, p < 0.0001). The prevalence of hypogonadism (overt + compensated) was 17.1% for cFT using LC-MS/MS. Secondary form of hypogonadism was more prevalent than primary. The prevalence of relative estrogen deficiency was of 30.0% among hypogonadal patients and 15.5% among eugonadal.

Conclusions: The prevalence of male hypogonadism results underestimated by CI compared to LC-MS/MS in PLWH, both for TT and cFT. SHBG and gonadotropins are essential for detecting T deficiency.

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HIV感染者原发性、继发性和代偿性男性生化性腺功能减退(PLWH):性激素结合球蛋白(SHBG)测定的相关性以及液相色谱-串联质谱(LC-MS/MS)和化学发光免疫分析法在性激素测定中的比较

背景:关于男性HIV感染者(PLWH)性腺功能减退和雌激素缺乏的分类资料很少。目的:通过液相色谱-串联质谱法(LC-MS/MS)和化学发光免疫分析法(CI)，结合促性腺激素、性激素结合球蛋白(SHBG)和血清雌二醇(E2)检测，探讨50岁以下男性PLWH中生化性腺功能减退和相对雌激素缺乏的患病率及特征。方法:前瞻性、横断面、观察性研究。CI法测定血清总睾酮(TT)、E2、促性腺激素、SHBG。采用LC-MS/MS法测定TT和E2。用Vermeulen方程计算游离睾酮(cFT)。结果:共纳入316例PLWH患者(45.3±5.3岁)。结论:与LC-MS/MS相比，CI低估了PLWH中男性性腺功能减退的患病率，无论是TT还是cFT。SHBG和促性腺激素是检测T缺乏所必需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.