Evaluation of Toxicological Effects of ZnO and CuO Nanoparticles with Taraxacum officinale as Bioindicator

IF 3.8 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2023-07-01 DOI:10.1007/s11270-023-06432-3

Paulina Abrica-González, Sandra Gómez-Arroyo, Arón Jazcilevich-Diamant, Antonio Sotelo-López, Ana Rosa Flores-Márquez, Josefina Cortés-Eslava

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引用次数: 1

Abstract

Nanoparticles are rising worries because of recent reports about potential toxicity amid the incorporation of these emerging materials into consumer products, and industrial and scientific applications. New developments in the automotive industry are incorporating novel materials, which have increased the emission of nanoparticles into the atmosphere. To overcome the difficulty of detecting and characterizing atmospheric nanoparticles, alternative methods have been proposed, just as the indirect detection and characterization with bioindicators. We report the use of Taraxacum officinale as a sentinel organism to describe the effects of atmospheric nanostructured pollutants. ZnO and CuO nanoparticles (ZnO-NPs, CuO-NPs) were selected for this study, as they are two of the most present nanomaterials in the emerging automotive industry. The physiological effect on Taraxacum officinale exposure to ZnO-NPs and CuO-NPs was evaluated through growth rate, and total chlorophyll content; and comet assay was performed to evaluate the DNA damage. The exposure of plants was made by nebulizing dispersions of the nanoparticles. The exposure to ZnO-NPs presented the maximum DNA damage at a concentration of 100 mg/L. The DNA damage by both studied nanoparticles showed a significant difference against its bulk counterparts. Scanning electron microscopy (SEM) micrographs showed an accumulation of nanoparticles near the stomata. The study demonstrated the feasibility of T. officinale as a bioindicator of air-related nanoparticles toxicity, and the high sensitivity of the comet assay for this approach.

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以蒲公英为生物指示剂评价ZnO和CuO纳米颗粒的毒理学效应

摘要纳米粒子引起越来越多的担忧，因为最近有报道称，在将这些新兴材料纳入消费品以及工业和科学应用中，它们可能具有毒性。汽车工业的新发展正在采用新材料，这增加了向大气中排放纳米颗粒。为了克服大气纳米粒子检测和表征的困难，人们提出了一些替代方法，如生物指示剂的间接检测和表征。我们报道了使用蒲公英作为哨兵生物来描述大气纳米结构污染物的影响。本研究选择ZnO和CuO纳米颗粒(ZnO- nps, CuO- nps)，因为它们是新兴汽车工业中最常见的两种纳米材料。通过生长速率和总叶绿素含量评价ZnO-NPs和CuO-NPs对蒲公英的生理影响;采用彗星法评估DNA损伤。植物暴露是通过雾化纳米粒子的分散体来实现的。ZnO-NPs浓度为100 mg/L时DNA损伤最大。两种研究的纳米颗粒对DNA的损伤表现出显著的差异。扫描电镜(SEM)显示气孔附近有纳米颗粒堆积。该研究证明了officinale作为空气相关纳米颗粒毒性生物指示剂的可行性，以及彗星试验对该方法的高灵敏度。

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.