1H, 15N and 13C resonance assignments of a minimal CPSF73-CPSF100 C-terminal heterodimer

IF 0.8 4区 生物学 Q4 BIOPHYSICS
Stéphane Thore, Sébastien Fribourg, Cameron D. Mackereth
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引用次数: 1

Abstract

The initial pre-mRNA transcript in eukaryotes is processed by a large multi-protein complex in order to correctly cleave the 3’ end, and to subsequently add the polyadenosine tail. This cleavage and polyadenylation specificity factor (CPSF) is composed of separate subunits, with structural information available for both isolated subunits and also larger assembled complexes. Nevertheless, certain key components of CPSF still lack high-resolution atomic data. One such region is the heterodimer formed between the first and second C-terminal domains of the endonuclease CPSF73, with those from the catalytically inactive CPSF100. Here we report the backbone and sidechain resonance assignments of a minimal C-terminal heterodimer of CPSF73–CPSF100 derived from the parasite Encephalitozoon cuniculi. The assignment process used several amino-acid specific labeling strategies, and the chemical shift values allow for secondary structure prediction.

Abstract Image

最小CPSF73-CPSF100 c端异二聚体的1H, 15N和13C共振分配
真核生物中最初的pre-mRNA转录物由一个大的多蛋白复合物加工,以正确地切割3 '端,并随后添加聚腺苷尾部。这种切割和聚腺苷酸化特异性因子(CPSF)由独立的亚基组成,其结构信息可用于分离的亚基和更大的组装复合物。然而,CPSF的某些关键组件仍然缺乏高分辨率的原子数据。其中一个区域是在内切酶CPSF73的第一和第二c端结构域之间形成的异二聚体,这些区域来自催化活性不强的CPSF100。在这里,我们报道了CPSF73-CPSF100的最小c端异二聚体的主链和侧链共振分配。分配过程使用了几种氨基酸特异性标记策略,化学位移值允许二级结构预测。
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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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