A tri-fluid tortuous microfluidic chip for green synthesis of nanoparticles and inactivation of a model gram-negative bacteria: Intracellular components evaluation

IF 2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Arash Haghighinia, Salman Movahedirad
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引用次数: 1

Abstract

The development of green synthesis route using plant extract as a simple, cost-effective, and eco-friendly method for the synthesis of nanoparticles has become a major focus of researchers in recent years. In the present study, a novel continuous tri-fluid tortuous microfluidic chip (CTTM) was constructed to induce simultaneous mixing, Dean vortices, tortuosity, and repetitive bending in fluid behavior in order to plant-mediated synthesis of zinc selenide (ZnSe) nanoparticles. Additionally, the anti-pathogenic activity of nanoparticles against a human pathogen (E. coli) through the disruption of the cell membrane and the evaluation of the subsequent flow of cellular components such as continuous leakages of K+, nucleic acid, and intracellular protein was examined using the proposed chip. According to the results, by changing the flow rates up to 1.50 mL/min, nanoparticles with narrow size distribution were obtained. It was found that the nanoparticles sterilization effect in the case of α (Vnanoparticles/Vbacteria strain) =2 was obviously better than α = 0.5 under similar concentration and culture conditions. In this case, when the residence time and nanoparticle concentration tended to the maximum values, the release of intracellular components increased. Light microscopy and SEM clearly confirmed the ability of the antibacterial effects of nanoparticles to disrupt the bacteria membrane. Moreover, the inhibitory activity of the fabricated nanoparticles through a protein denaturation test using human serum albumin (HSA) showed an acceptable ability to inhibit protein denaturation compared to the inhibition of diclofenac sodium as a standard anti-inflammatory drug at the same concentration.

Abstract Image

用于绿色合成纳米颗粒和模型革兰氏阴性细菌灭活的三流体弯曲微流控芯片:胞内成分评估
以植物提取物为原料,开发一种简单、经济、环保的合成纳米颗粒的绿色合成途径是近年来研究人员关注的热点。在本研究中,构建了一种新型的连续三流体扭曲微流控芯片(CTTM),以诱导流体行为的同步混合、迪安涡流、扭曲和重复弯曲,以实现植物介导的硒化锌(ZnSe)纳米颗粒的合成。此外,纳米颗粒对人类病原体(大肠杆菌)的抗致病活性通过破坏细胞膜和评估随后的细胞成分流动,如K+、核酸和细胞内蛋白的连续泄漏,使用所提出的芯片进行了检测。结果表明,当流速达到1.50 mL/min时,可获得粒径分布较窄的纳米颗粒。结果表明,在相同浓度和培养条件下,当α (v纳米颗粒/ v菌株)=2时,纳米颗粒的杀菌效果明显优于α = 0.5。在这种情况下,当停留时间和纳米颗粒浓度趋于最大值时,细胞内成分的释放量增加。光镜和扫描电镜清楚地证实了纳米颗粒破坏细菌膜的抗菌作用。此外,通过使用人血清白蛋白(HSA)进行的蛋白质变性试验,制备的纳米颗粒的抑制活性显示,与双氯芬酸钠作为标准抗炎药在相同浓度下的抑制作用相比,抑制蛋白质变性的能力是可以接受的。
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来源期刊
Journal of Flow Chemistry
Journal of Flow Chemistry CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
6.40
自引率
3.70%
发文量
29
审稿时长
>12 weeks
期刊介绍: The main focus of the journal is flow chemistry in inorganic, organic, analytical and process chemistry in the academic research as well as in applied research and development in the pharmaceutical, agrochemical, fine-chemical, petro- chemical, fragrance industry.
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