{"title":"Expression of IL-17 and its role in bone destruction in human middle ear cholesteatoma.","authors":"Takuo Haruyama, Masayuki Furukawa, Takeshi Kusunoki, Junko Onoda, Katsuhisa Ikeda","doi":"10.1159/000319897","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The present study was designed to elucidate the immunoreactivity and protein level of IL-17 in human cholesteatomas.</p><p><strong>Procedures: </strong>The expression and localization of IL-17 and receptor activator of nuclear factor κB ligand (RANKL) were examined by immunohistochemistry in tissue specimens collected from 24 patients with cholesteatomas. The cellular sources of IL-17 were assessed by double staining with CD4. The level of IL-17 protein was determined using an enzyme-linked immunosorbent assay. The degree of bone destruction was compared with the IL-17 immunoreactivity.</p><p><strong>Results: </strong>IL-17 immunoreactivity was detected in the inflammatory cells in the granulation tissues and there were increased levels of IL-17 protein. The localization of IL-17 expression coincided with CD4-positive lymphocytes. IL-17 was correlated with the cells positive for RANKL. The degree of bone destruction was dependent on the number of infiltrated IL-17-positive cells. IL-17-driven pathology was observed in the congenital type as compared with the acquired type.</p><p><strong>Conclusions: </strong>The present study suggests that IL-17 is related to the pathogenesis of the disease.</p>","PeriodicalId":520736,"journal":{"name":"ORL; journal for oto-rhino-laryngology and its related specialties","volume":" ","pages":"325-31"},"PeriodicalIF":1.3000,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000319897","citationCount":"19","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ORL; journal for oto-rhino-laryngology and its related specialties","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000319897","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2010/9/16 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 19
Abstract
Purpose: The present study was designed to elucidate the immunoreactivity and protein level of IL-17 in human cholesteatomas.
Procedures: The expression and localization of IL-17 and receptor activator of nuclear factor κB ligand (RANKL) were examined by immunohistochemistry in tissue specimens collected from 24 patients with cholesteatomas. The cellular sources of IL-17 were assessed by double staining with CD4. The level of IL-17 protein was determined using an enzyme-linked immunosorbent assay. The degree of bone destruction was compared with the IL-17 immunoreactivity.
Results: IL-17 immunoreactivity was detected in the inflammatory cells in the granulation tissues and there were increased levels of IL-17 protein. The localization of IL-17 expression coincided with CD4-positive lymphocytes. IL-17 was correlated with the cells positive for RANKL. The degree of bone destruction was dependent on the number of infiltrated IL-17-positive cells. IL-17-driven pathology was observed in the congenital type as compared with the acquired type.
Conclusions: The present study suggests that IL-17 is related to the pathogenesis of the disease.
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