An experiment of the combined treatment of traditional Lei-huo-jiu therapy with Chinese medicine for the lacrimal gland of Sjögren's syndrome.

Abstract

This experiment chooses nonobese diabetic (NOD) mouse as the animal model of Sjögren's syndrome and investigates the morphologic changes, the expression of inflammatory factors and growth factors of this mouse's lacrimal gland in response to a combined treatment of traditional Lei-huo-jiu therapy alone and in combination with Chinese medicine. The methods were to (1) use a morphological approach to directly observe pathological changes of the lacrimal gland in response to combined treatment and (2) to detect the level of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1, and nuclear factor kappa B (NF-kappaB) in lacrimal gland tissue caused by the combined treatments using a immunohistochemical approach. There is a reduction of the mast cell's degranulation and modulation of the level of cytokines in TNF-alpha, IL-1, and NF-kappaB in the combined therapy group. The combined treatment of traditional Lei-huo-jiu therapy with Chinese medicine can improve the pathological changes of the lacrimal gland tissue of the NOD mouse through modulating the level of TNF-alpha, IL-1, and NF-kappaB which results in improved tear secretion and function of the lacrimal gland.