Shotgun proteomic investigation of methyltransferase and methylation profiles in lipopolysaccharide stimulated RAW264.7 murine macrophages.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Yumi Aizawa, Masaru Mori, Tsukasa Suzuki, Akihiro Saito, Hirofumi Inoue
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引用次数: 1

Abstract

Arginine methylation is a common post-translational modification which functions as an epigenetic regulator of transcription and plays a key role in various cell signaling pathways. The methylation of arginine residues is catalyzed by protein arginine methyltransferase (PRMT). However, the expression pattern and underlying mechanism of PRMTs and protein methylation profile in lipopolysaccharide (LPS)-induced innate immune responses are poorly understood. Using a shotgun proteomic approach, we found that LPS stimulation increased arginine and proline metabolism and responses to inflammation and bacterial infections. In comparison, cysteine and methionine metabolism, the pentose phosphate pathway, purine metabolism, and protein methylation factors were also decreased in LPS stimulated murine macrophage cell lines. We revealed that LPS stimulation downregulated PRMT1, PRMT5, and protein arginine methylation profiles in RAW264.7 cells using western blot analysis. Additionally, this phenomenon occurred in parallel with nitric oxide accumulation in LPS-induced macrophages. Using inflammation models, we demonstrate for the first time that LPS stimulation decreases PRMTs, leading to the decreasing of arginine methylation in macrophages.

脂多糖刺激RAW264.7小鼠巨噬细胞中甲基转移酶和甲基化谱的散弹枪蛋白质组学研究。
精氨酸甲基化是一种常见的翻译后修饰,是一种转录的表观遗传调控因子,在多种细胞信号通路中起着关键作用。精氨酸残基的甲基化是由蛋白精氨酸甲基转移酶(PRMT)催化的。然而,在脂多糖(LPS)诱导的先天免疫应答中,PRMTs的表达模式和潜在机制以及蛋白甲基化谱尚不清楚。使用散弹枪蛋白质组学方法,我们发现LPS刺激增加了精氨酸和脯氨酸代谢以及对炎症和细菌感染的反应。相比之下,LPS刺激小鼠巨噬细胞的半胱氨酸和蛋氨酸代谢、戊糖磷酸途径、嘌呤代谢和蛋白质甲基化因子也降低。通过western blot分析,我们发现LPS刺激下调了RAW264.7细胞中PRMT1、PRMT5和蛋白精氨酸甲基化谱。此外,这种现象与lps诱导的巨噬细胞中一氧化氮的积累同时发生。通过炎症模型,我们首次证明LPS刺激降低PRMTs,导致巨噬细胞精氨酸甲基化降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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