An enhancer trap zebrafish line for lateral line development and regulation of six2b expression

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2022-03-01 DOI:10.1016/j.gep.2022.119231

Chunxin Fan , Yajing Ouyang , Xiaoyi Yuan , Jian Wang

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Abstract

Zebrafish lateral line system which is derived from neurogenic placodes has become a popular model for developmental biology since its formation involves cell migration, pattern formation, organogenesis, and hair cell regeneration. Transgenic lines play a crucial role in lateral line system study. Here, we identified an enhancer trap transgenic zebrafish line Et(gata2a:EGFP)189b (ET189b for short), which expressed enhanced green fluorescent protein (EGFP) in the pituitary, otic, and lateral line placodes and their derivatives. Especially, in neuromast, the accessory cells rather than hair cells were labeled by EGFP. Furthermore, we found the Tol2 transposon construct is integrated at the proximal upstream region of six2b gene locus. And EGFP expression of ET189b closely reflects the expression of endogenous six2b during development and after dkk1b over-expression. Taken together, our results indicated that ET189b is an ideal line for research on lateral line development and regulation of six2b expression.

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一种增强子诱捕斑马鱼系用于侧线发育和six2b表达调控

斑马鱼侧线系统起源于神经源性基板，其形成涉及细胞迁移、模式形成、器官发生和毛细胞再生，已成为发育生物学研究的热门模型。转基因系在侧线系研究中起着至关重要的作用。在这里，我们发现了一个增强子陷阱转基因斑马鱼系Et(gata2a:EGFP)189b(简称ET189b)，它在垂体、耳部和侧线基板及其衍生物中表达增强的绿色荧光蛋白(EGFP)。尤其在神经肥大中，EGFP标记的是副细胞而不是毛细胞。此外，我们发现Tol2转座子结构整合在six2b基因位点的近上游区域。ET189b的EGFP表达密切反映了内源性six2b在发育期间和dkk1b过表达后的表达。综上所述，我们的结果表明，ET189b是研究侧线发育和six2b表达调控的理想细胞系。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation