Study design, general characteristics of participants, and preliminary findings from the metabolome, microbiome, and dietary salt intervention study (MetaSalt)

Q1 Medicine

Chronic Diseases and Translational Medicine Pub Date : 2021-12-01 DOI:10.1016/j.cdtm.2021.06.002

Zengliang Ruan , Jianxin Li , Fangchao Liu , Jie Cao , Shufeng Chen , Jichun Chen , Keyong Huang , Yaqin Wang , Hongfan Li , Yan Wang , Zhongyu Xue , Laiyuan Wang , Jianfeng Huang , Dongfeng Gu , Xiangfeng Lu

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引用次数: 0

Abstract

Background

High sodium intake is an important risk factor for hypertension and cardiovascular disease. However, the association between gut microbiota composition and metabolomic profiles with dietary sodium intake and blood pressure (BP) is not well-understood. The metabolome, microbiome, and dietary salt intervention (MetaSalt) study aimed to investigate microbial and metabolomic profiles related to dietary sodium intake and BP regulation.

Methods

This family-based intervention study was conducted in four communities across three provinces in rural northern China in 2019. Probands with untreated prehypertension or stage-1 hypertension were identified through community-based BP screening, and family members including siblings, offspring, spouses, and parents were subsequently included. All participants participated in a 3-day baseline examination with usual diet consumption, followed by a 10-day low-salt diet (3 g/d of salt or 51.3 mmol/d of sodium) and a 10-day high-salt diet (18 g/d of salt or 307.8 mmol/d of sodium). Differences in mean BP levels were compared according to the intervention phases using a paired Student's t-test.

Results

A total of 528 participants were included in this study, with a mean age of 48.1 years, 36.7% of whom were male, 76.8% had a middle school (69.7%) or higher (7.1%) diploma, 23.4% had a history of smoking, and 24.4% were current drinkers. The mean arterial pressure at baseline was 97.2 ± 10.5 mm Hg for all participants, and significantly decreased during the low-salt intervention (93.8 ± 9.3, P < 0.0001) and subsequently increased during the high-salt intervention (96.4 ± 10.0, P < 0.0001).

Conclusions

Our dietary salt intervention study has successfully recruited participants and will facilitate to evaluate the effects of gut microbiota and metabolites on BP regulation in response to sodium burden, which will provide important evidence for investigating the underlying mechanisms in the development of hypertension and subsequent cardiovascular diseases.

Trial registration

The study was registered in the Chinese Clinical Trial Registry database (ChiCTR1900025171).

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研究设计、参与者的一般特征以及代谢组、微生物组和饮食盐干预研究(MetaSalt)的初步发现

背景高钠摄入是高血压和心血管疾病的重要危险因素。然而，肠道微生物群组成和代谢组学特征与膳食钠摄入量和血压(BP)之间的关系尚不清楚。代谢组、微生物组和膳食盐干预(MetaSalt)研究旨在研究与膳食钠摄入和血压调节相关的微生物和代谢组学特征。方法2019年在中国北方农村3省4个社区开展以家庭为基础的干预研究。通过基于社区的血压筛查确定未经治疗的高血压前期或1期高血压的先证者，随后纳入家庭成员，包括兄弟姐妹、后代、配偶和父母。所有参与者都参加了为期3天的基线检查，正常饮食，随后是10天的低盐饮食(3 g/d盐或51.3 mmol/d钠)和10天的高盐饮食(18 g/d盐或307.8 mmol/d钠)。采用配对学生t检验比较不同干预阶段的平均血压水平差异。结果共纳入528名参与者，平均年龄48.1岁，男性占36.7%，76.8%(69.7%)具有中学及以上学历(7.1%)，23.4%有吸烟史，24.4%有饮酒者。所有参与者基线时的平均动脉压为97.2±10.5 mm Hg，在低盐干预期间显著降低(93.8±9.3,P <0.0001)，随后在高盐干预期间增加(96.4±10.0,P <0.0001)。结论我们的饮食盐干预研究成功招募了参与者，将有助于评估肠道微生物群和代谢物在钠负荷下对血压调节的影响，为探讨高血压和心血管疾病发生的潜在机制提供重要证据。该研究已在中国临床试验注册数据库(ChiCTR1900025171)中注册。

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来源期刊

Chronic Diseases and Translational Medicine Medicine-General Medicine

CiteScore

6.70

自引率

0.00%

发文量

195

审稿时长

35 weeks

期刊介绍： This journal aims to promote progress from basic research to clinical practice and to provide a forum for communication among basic, translational, and clinical research practitioners and physicians from all relevant disciplines. Chronic diseases such as cardiovascular diseases, cancer, diabetes, stroke, chronic respiratory diseases (such as asthma and COPD), chronic kidney diseases, and related translational research. Topics of interest for Chronic Diseases and Translational Medicine include Research and commentary on models of chronic diseases with significant implications for disease diagnosis and treatment Investigative studies of human biology with an emphasis on disease Perspectives and reviews on research topics that discuss the implications of findings from the viewpoints of basic science and clinical practic.