Human Hepatocytes Isolated from Explanted Livers: A Powerful Tool to Understand End-stage Liver Disease and Drug Screening.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2021-10-02 DOI:10.1080/15476278.2021.1992216

Lanuza A P Faccioli, Zehra N Kocas-Kilicarslan, Ricardo Diaz-Aragon, Takashi Motomura, Sriram Amirneni, Michelle R Malizio, Michael C Coard, Carla Frau, Nils Haep, Rodrigo M Florentino, Alina Ostrowska

引用次数: 1

Abstract

The use of primary human hepatocytes has been hampered by limited availability of adequate numbers of fresh and viable cells due to the ongoing shortage of liver donors. Thus, there is no surplus of healthy organs from which freshly isolated cells can be prepared when needed. However, primary hepatocytes can be successfully isolated from explanted liver specimens obtained from patients receiving orthotopic liver transplantation for decompensated liver cirrhosis or for metabolic liver disease without end-stage liver disease and are a valuable resource for the pharmaceutical industry research. This review focuses on the isolation, characterization and cryopreservation of hepatocytes derived from therapeutically resected livers with various hepatic diseases.

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从外植肝脏中分离的人肝细胞:了解终末期肝病和药物筛选的有力工具。

由于肝脏供体持续短缺，新鲜和活细胞数量有限，阻碍了原代人肝细胞的使用。因此，没有多余的健康器官可以在需要时从中制备新鲜分离的细胞。然而，原代肝细胞可以成功地从接受原位肝移植的失代偿肝硬化或代谢性肝病无终末期肝病患者的外植肝标本中分离出来，是制药行业研究的宝贵资源。本文综述了各种肝脏疾病治疗性切除肝脏中肝细胞的分离、表征和冷冻保存。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.