Cytological Screening Model of Normal Oral Mucosa Exposed to Carcinogens: A Pilot Study.

IF 1.6 4区医学 Q3 PATHOLOGY

Acta Cytologica Pub Date : 2022-01-01 Epub Date: 2021-11-16 DOI:10.1159/000519877

Ricardo Losekann Paiva, Maria Antonia Zancanaro de Figueiredo, Karen Cherubini, Vinicius Duval Da Silva, Fernanda Gonçalves Salum

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引用次数: 1

Abstract

Introduction: Oral cytopathology is able to detect incipient cellular alterations, but it is not routinely applied to this purpose. We aimed to establish a model to screen individuals with no oral lesion exposed to smoking/alcohol, by means of the nuclear area, cell proliferation rate, and analysis of genetic damage.

Methods: In this cross-sectional pilot study, 90 patients were allocated into 3 groups: oral cancer group (patients with oral squamous cell carcinoma), tobacco/alcohol group (patients without oral lesions and exposed to these risk factors), and control group (individuals with no lesion and not exposed to tobacco and alcohol). The cytological smears performed in these individuals were stained with Papanicolaou, a silver-staining and a Feulgen reaction. The nuclei of cells were measured, and AgNORs/nucleus and micronuclei (MN) were quantified. The cutoff values were stipulated evaluating the healthy mucosa (control group) and the cancerization field mucosa (oral cancer group).

Results: Cutoff values for the screening of individuals exposed to carcinogens were ≥8% of nuclei larger than 100 μm2, ≥3.38 AgNOR/nucleus, and ≥3 MN per 1,000 cells.

Conclusions: Nuclear area measurement and AgNORs/nucleus and MN quantification identified the incipient phase of oral carcinogenesis. A screening model for individuals without oral lesion exposed to smoking/alcohol was proposed.

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正常口腔黏膜暴露于致癌物的细胞学筛选模型:一项初步研究。

口腔细胞病理学能够检测早期的细胞改变，但它不是常规应用于此目的。我们的目的是建立一个模型，通过核面积、细胞增殖率和基因损伤分析来筛选无口腔病变暴露于吸烟/酒精的个体。方法:本横断面先导研究将90例患者分为3组:口腔癌组(口腔鳞状细胞癌患者)、烟酒组(口腔无病变且暴露于上述危险因素的患者)和对照组(无病变且未暴露于烟酒环境的患者)。在这些个体中进行细胞学涂片，用帕帕尼科劳染色，银染色和Feulgen反应染色。测定细胞核，定量测定AgNORs/细胞核和微核(MN)。分别以健康粘膜(对照组)和癌变场粘膜(口腔癌组)为评价标准，设定临界值。结果:暴露于致癌物个体的筛选临界值为≥8%大于100 μm2的细胞核，≥3.38 AgNOR/细胞核，≥3 MN / 1000个细胞。结论:核面积测量和AgNORs/核和MN定量鉴定了口腔癌变的早期阶段。提出了一种无口腔损伤吸烟/酒精暴露个体的筛查模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Cytologica 生物-病理学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

4-8 weeks

期刊介绍： With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.