Evaluation and comparison of cytotoxicity and bioactivity of chemomechanical caries removal agents on stem cells from human exfoliated deciduous teeth.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry Pub Date : 2022-10-01 Epub Date: 2021-11-12 DOI:10.1007/s40368-021-00684-5

V Maru, M Madkaikar, S Shabrish, P Kambli, A Dalvi, P Setia

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引用次数: 2

Abstract

Aim: To investigate and compare the cytotoxicity and bioactivity of CMCR agents on stem cells derived from exfoliated deciduous teeth.

Methodology: MTT assay, flow cytometry, Alizarin Red staining and scratch assay were used to assess the cellular viability, apoptosis, calcium matrix deposits and cell migration, respectively. The gene expression of ALP and BMP-2 was measured with RT-PCR. One-way ANOVA and Bonferroni post-test was used for statistical analysis.

Results: 0.5% Carisolv showed highest cell proliferation and calcium matrix formation, whereas 0.5% Papacarie reported the highest% live cells and cell migration. The highest mRNA expression of ALP and BMP-2 was reported in SHEDs cultured in 0.5% Papacarie (after 72 h incubation) and 0.5% Carisolv (after 24 h incubation), respectively.

Conclusion: CMCR agents are biocompatible and bioactive when cultured in stem cells derived from exfoliated primary teeth.

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化学力学除龋剂对人脱落乳牙干细胞的细胞毒性和生物活性的评价与比较。

目的:研究和比较CMCR制剂对脱落乳牙干细胞的细胞毒性和生物活性。方法:分别采用MTT法、流式细胞术、茜素红染色法和划痕法测定细胞活力、细胞凋亡、钙基质沉积和细胞迁移。RT-PCR检测ALP、BMP-2基因表达。采用单因素方差分析和Bonferroni后验进行统计分析。结果:0.5% Carisolv具有最高的细胞增殖率和钙基质形成率，0.5% Papacarie具有最高的活细胞率和细胞迁移率。ALP和BMP-2 mRNA在0.5% Papacarie(孵育72 h)和0.5% Carisolv(孵育24 h)培养液中表达量最高。结论:CMCR制剂在原牙脱落干细胞中培养具有生物相容性和生物活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry

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