Factors necessary to secure stem cell stability

Abstract

Obtaining stable embryonic stem cells (ESC) from animals and humans opens up a wide spectrum of opportunities for science and regenerative medicine. The basic procedures necessary to obtain ESC are universal for all mammalian species. The challenge is to maintain species specific conditions required to support pluripotency characteristic for the pre-implantation embryo. To date, true ESC lines (stable in culture, pluripotent with high differentiation potential) have been obtained only for a limited number of species such as, mice, human and rats. The main obstacles arise from species specific differences, and thus different environmental requirements. However, in all cases, it is essential to maintain the activity of pluripotency related signalling pathways (WNT, MAPK/ERK and JAK/STAT3). The classical system dedicated to obtain mouse and human ESCs dependant on LIF (mice) of FGF (human) is not optimal. Currently, ESC derivation systems are based on chemical inhibitors that have the ability to interact with the above-mentioned pathways. This manuscript introduces the key factors important for understanding the nature of various types of stem cells (not only those of embryonic origin), and explains why there is no one way to obtain pluripotency, and why the definition of a stem cell is not universal.