Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae).

IF 1 4区生物学 Q4 GENETICS & HEREDITY

Comparative Cytogenetics Pub Date : 2021-12-17 eCollection Date: 2021-01-01 DOI:10.3897/compcytogen.v15.i4.75990

Alessio Turco, Antonella Albano, Pietro Medagli, Robert P Wagensommer, Saverio D'Emerico

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引用次数: 3

Abstract

Abstract In our study, FISH mapping using 18S-5.8S-25S rDNA and 5S rDNA sequences was performed for the first time on Ophrystenthredinifera Willdenow, 1805, Serapiasvomeracea (Burman f., 1770) Briquet, 1910 and Himantoglossumhircinum (Linnaeus, 1753) Sprengel, 1826. A detailed study was also performed on O.tenthredinifera using Giemsa-staining, silver-staining, CMA fluorescence banding and fluorescence in situ hybridisation (FISH) with rDNA probes. We analysed two subspecies, i.e. O.tenthrediniferasubsp.neglecta (Parlatore, 1860) E.G. Camus, 1908 and O.tenthrediniferasubsp.grandiflora (Tenore, 1819) Kreutz, 2004 by the traditional Feulgen method and constructed the karyotype. The cytotaxonomic implications for both taxa are also discussed. In Himantoglossumhircinum, FISH and silver staining highlighted differences in the number of two rDNA families (35S and 5S) with respect to Barliarobertiana (Loiseleur-Deslongchamps, 1807) Greuter, 1967. In addition, fluorescence in situ hybridisation was also applied to diploid (2n = 2x = 36) and triploid (2n = 3x = 54) Anacamptismorio (Linnaeus, 1753) R.M. Bateman, Pridgeon et M.W. Chase, 1997. As far as we are aware, this is the first case of autotriploidy observed in A.morio.

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Comparative兰科兰科亚族种的染色体研究。

本研究首次利用18S-5.8S-25S rDNA和5S rDNA序列对Ophrystenthredinifera Willdenow(1805)、Serapiasvomeracea (Burman f.， 1770) Briquet(1910)和Himantoglossumhircinum (Linnaeus, 1753) Sprengel(1826)进行FISH定位。采用giemsa染色、银染色、CMA荧光带和荧光原位杂交(FISH)等方法，用rDNA探针对黄颡鱼进行了详细的研究。采用传统的Feulgen方法分析了两个亚种，即o.tenthrediniferasubsp .忽略ta (Parlatore, 1860) E.G. Camus, 1908和o.tenthrediniferasubsp . grandflora (Tenore, 1819) Kreutz, 2004，并构建了核型。对这两个类群的细胞分类意义也进行了讨论。在Himantoglossumhircinum中，与Barliarobertiana (Loiseleur-Deslongchamps, 1807)相比，FISH和银染色突出了两个rDNA家族(35S和5S)的数量差异。此外，荧光原位杂交也应用于二倍体(2n = 2x = 36)和三倍体(2n = 3x = 54) Anacamptismorio (Linnaeus, 1753) R.M. Bateman, Pridgeon et M.W. Chase, 1997。据我们所知，这是在A.morio中观察到的第一例自体三倍体。

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来源期刊

Comparative Cytogenetics 生物-遗传学

CiteScore

2.40

自引率

0.00%

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审稿时长

>12 weeks

期刊介绍： Comparative Cytogenetics is a peer-reviewed, open-access, rapid online journal launched to accelerate research on all aspects of plant and animal cytogenetics, karyosystematics, and molecular systematics. All published papers can be freely copied, downloaded, printed and distributed at no charge for the reader. Authors are thus encouraged to post the pdf files of published papers on their homepages or elsewhere to expedite distribution. There is no charge for color.

﻿Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae).

Abstract

Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae).