Sodium Alginate versus Plasma Thrombin Cell Blocks in Diagnostic Cytopathology: A Comparative Analysis.

IF 1.6 4区医学 Q3 PATHOLOGY

Acta Cytologica Pub Date : 2022-01-01 Epub Date: 2021-10-29 DOI:10.1159/000519336

Shruti Gupta, Upasana Gautam, Shaily Susheilia, Baneet Bansal, Radha Uppal, Radhika Srinivasan

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引用次数: 1

Abstract

Background: Cell blocks (CBs) are an essential adjunct in cytopathology practice. The aim of this study was to compare 2 techniques of CB preparation - plasma thrombin (PT) method with sodium alginate (SA) method for overall cellularity, morphological preservation, obscuring artefacts, immunocytochemistry (ICC), suitability for molecular analysis, and cost of preparation.

Design: A total of 80 fine-needle aspirates from various sites and serous effusion samples were included. Of these cases, by random selection, 40 each were prepared by PT method and SA methods, respectively. The haematoxylin-eosin-stained sections from the formalin-fixed, paraffin-embedded CBs from both methods were evaluated in a blinded fashion by 2 cytopathologists and scored for cellularity, artefacts, and morphological preservation and analysed by χ2 test with Yates correction. We evaluated 6 cases from each method by ICC for a range of membrane, cytoplasmic and nuclear marker expression. DNA was extracted from four cases to evaluate their utility for molecular analysis.

Results: CB sections from PT and SA techniques showed comparable cellularity and excellent cytomorphological preservation. Blue gel-like artefacts were common in the SA technique but did not interfere with morphological evaluation. ICC staining results were also similar. DNA yield and utility for PCR were also comparable. The SA-CB cost half that of PT-CB (USD 0.4 vs. USD 1).

Conclusion: SA technique of CB preparation is an excellent low-cost alternative to PT method for CB preparation.

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海藻酸钠与血浆凝血酶细胞阻滞在细胞病理学诊断中的比较分析。

背景:细胞阻滞(CBs)是细胞病理学实践中必不可少的辅助手段。本研究的目的是比较血浆凝血酶(PT)法和海藻酸钠(SA)法两种制备方法在整体细胞结构、形态学保存、伪影模糊、免疫细胞化学(ICC)、分子分析适用性和制备成本等方面的差异。设计:共包括来自不同部位的80个细针抽吸器和浆液样本。随机选取PT法和SA法各制备40例。2名细胞病理学家采用盲法对两种方法制备的福尔马林固定石蜡包埋CBs的血丝精-伊红染色切片进行评估，对细胞结构、伪影和形态学保存进行评分，并采用χ2检验和Yates校正进行分析。我们用ICC对每种方法的6例进行了膜、细胞质和核标记物表达范围的评估。从四个病例中提取DNA以评估其在分子分析中的效用。结果:PT和SA技术的CB切片显示相当的细胞结构和良好的细胞形态学保存。蓝色凝胶样伪影在SA技术中很常见，但不会干扰形态学评价。ICC染色结果也相似。PCR的DNA产量和效用也具有可比性。SA-CB的成本是PT-CB的一半(0.4美元比1美元)。结论:SA技术制备CB是一种极好的替代PT法制备CB的低成本技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Cytologica 生物-病理学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

4-8 weeks

期刊介绍： With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.