Kevin Wang, Karissa J. Muñoz, Ming Tan, Christine Sütterlin
{"title":"Chlamydia and HPV induce centrosome amplification in the host cell through additive mechanisms","authors":"Kevin Wang, Karissa J. Muñoz, Ming Tan, Christine Sütterlin","doi":"10.1111/cmi.13397","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <p>Based on epidemiology studies, <i>Chlamydia trachomatis</i> has been proposed as a co-factor for human papillomavirus (HPV) in the development of cervical cancer. These two intracellular pathogens have been independently reported to induce the production of extra centrosomes, or centrosome amplification, which is a hallmark of cancer cells. We developed a cell culture model to systematically measure the individual and combined effects of <i>Chlamydia</i> and HPV on the centrosome in the same host cell. We found that <i>C. trachomatis</i> caused centrosome amplification in a greater proportion of cells than HPV and that the effects of the two pathogens on the centrosome were additive. Furthermore, centrosome amplification induced by <i>Chlamydia</i>, but not by HPV, strongly correlated with multinucleation and required progression through mitosis. Our results suggest that <i>C. trachomatis</i> and HPV induce centrosome amplification through different mechanisms, with the chlamydial effect being largely due to a failure in cytokinesis that also results in multinucleation. Our findings provide support for <i>C. trachomatis</i> as a co-factor for HPV in carcinogenesis and offer mechanistic insights into how two infectious agents may cooperate to promote cancer.</p>\n </section>\n \n <section>\n \n <h3> Take Aways</h3>\n \n <p>• <i>Chlamydia</i> and HPV induce centrosome amplification in an additive manner.</p>\n \n <p>• <i>Chlamydia</i>-induced centrosome amplification is linked to host cell multinucleation.</p>\n \n <p>• <i>Chlamydia</i>-induced centrosome amplification requires cell cycle progression.</p>\n \n <p>• <i>Chlamydia</i> and HPV cause centrosome amplification through different mechanisms.</p>\n \n <p>• This study supports <i>Chlamydia</i> as a co-factor for HPV in carcinogenesis.</p>\n </section>\n </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"23 12","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cmi.13397","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 7
Abstract
Based on epidemiology studies, Chlamydia trachomatis has been proposed as a co-factor for human papillomavirus (HPV) in the development of cervical cancer. These two intracellular pathogens have been independently reported to induce the production of extra centrosomes, or centrosome amplification, which is a hallmark of cancer cells. We developed a cell culture model to systematically measure the individual and combined effects of Chlamydia and HPV on the centrosome in the same host cell. We found that C. trachomatis caused centrosome amplification in a greater proportion of cells than HPV and that the effects of the two pathogens on the centrosome were additive. Furthermore, centrosome amplification induced by Chlamydia, but not by HPV, strongly correlated with multinucleation and required progression through mitosis. Our results suggest that C. trachomatis and HPV induce centrosome amplification through different mechanisms, with the chlamydial effect being largely due to a failure in cytokinesis that also results in multinucleation. Our findings provide support for C. trachomatis as a co-factor for HPV in carcinogenesis and offer mechanistic insights into how two infectious agents may cooperate to promote cancer.
Take Aways
• Chlamydia and HPV induce centrosome amplification in an additive manner.
• Chlamydia-induced centrosome amplification is linked to host cell multinucleation.
期刊介绍:
Cellular Microbiology aims to publish outstanding contributions to the understanding of interactions between microbes, prokaryotes and eukaryotes, and their host in the context of pathogenic or mutualistic relationships, including co-infections and microbiota. We welcome studies on single cells, animals and plants, and encourage the use of model hosts and organoid cultures. Submission on cell and molecular biological aspects of microbes, such as their intracellular organization or the establishment and maintenance of their architecture in relation to virulence and pathogenicity are also encouraged. Contributions must provide mechanistic insights supported by quantitative data obtained through imaging, cellular, biochemical, structural or genetic approaches.