Ethyl acetate extract of Clausena excavata induces growth inhibition of non-small-lung cancer, NCI-H460, cell line via apoptosis.

IF 2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Journal of applied biomedicine Pub Date : 2021-03-01 DOI:10.32725/jab.2021.007

Shaymaa Fadhel Abbas Albaayit, Mariam Ashfaq Khan, Rasedee Abdullah, Mohd Hezmee Mohd Noor

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引用次数: 10

Abstract

Context: Clausena excavata Burm. f is a plant used in folklore medicine for the treatment of various ailments in South East Asia. The plant parts contain chemical components that are cytotoxic to many cancer cells.

Objective: The study investigated the cytotoxic effects of ethyl acetate, methanol and chloroform C. excavata leaf extracts on the non-small-lung cancer, NCI-H460, cell line.

Methods: Based on the 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay, among extracts, ethyl acetate C. excavata leaf extract (EACE) was the most potent anti-NCI-H460 cells, with IC50 value of 47.1 ± 6.1 μg/ml. The effects of EACE on NCI-H460 cells were also determined by clonogenic, 4', 6-diamidino-2-phenylindole (DAPI), and annexin-V-fluorescein isothiocyanate/propidium iodide-PI flow cytometric assays. Reactive oxygen species (ROS) production and apoptotic gene expressions was determined via flow cytometry and real-time quantitative PCR, respectively.

Results: EACE-treated NCI-H460 cells after 48 h underwent apoptosis as evident by loss of cell viability, cell shrinkage, and chromatin condensation. The results also showed EACE mediated increase in ROS production by the NCI-H460 cells. After 48 h treatment, EACE increased the pro-apoptotic BAX and decreased the anti-apoptotic Bcl-2, Survivin and c-Myc gene expressions.

Conclusions: EACE is a potential anti-lung cancer by increasing cancer cell ROS production and apoptosis.

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竹叶草乙酸乙酯提取物通过凋亡诱导非小肺癌NCI-H460细胞株生长抑制。

背景:克劳塞纳(Clausena excavata burn)。它是东南亚民间医学中用于治疗各种疾病的植物。植物部分含有对许多癌细胞具有细胞毒性的化学成分。目的:研究乙酸乙酯、甲醇和氯仿提取物对非小肺癌NCI-H460细胞株的细胞毒作用。方法:采用3-(4,5-二甲基噻唑-2-基)-2,5，-二苯基溴化四唑(MTT)试验，结果表明，乙酸乙酯提取物(EACE)对nci - h460细胞的抑制作用最强，IC50值为47.1±6.1 μg/ml。通过克隆性、4′，6-二氨基-2-苯基吲哚(DAPI)和异硫氰酸膜联蛋白- v -荧光素/碘化丙啶- pi流式细胞术检测EACE对NCI-H460细胞的影响。通过流式细胞术和实时定量PCR分别检测活性氧(ROS)的产生和凋亡基因的表达。结果:eace处理的NCI-H460细胞在48 h后发生凋亡，表现为细胞活力丧失、细胞收缩和染色质凝聚。结果还显示EACE介导的NCI-H460细胞ROS生成增加。作用48 h后，EACE增加促凋亡BAX，降低抗凋亡Bcl-2、Survivin和c-Myc基因表达。结论:EACE通过增加肺癌细胞ROS生成和细胞凋亡，具有潜在的抗肺癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of applied biomedicine PHARMACOLOGY & PHARMACY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.