{"title":"Comparison of <sup>18</sup>F-FDG, <sup>18</sup>F-Fluoroacetate, and <sup>18</sup>F-FEPPA for Imaging Liver Fibrosis in a Bile Duct-Ligated Rat Model.","authors":"Chun-Yi Wu, Hsin-Hua Hsieh, Pei-An Chu, Wen-Hsiang Hong, Ting-Yu Chang, Chia-Fang Hsu, Siao-Ting Lin, Po-Hsun Su, Shin-Lei Peng","doi":"10.1155/2021/7545284","DOIUrl":null,"url":null,"abstract":"<p><p>Developing sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with <sup>18</sup>F-labeling tracers for assessing liver fibrosis in a rat model with bile duct ligation (BDL). Twenty-one 6-week-old Sprague-Dawley male rats were used in this study. Longitudinal PET images using [<sup>18</sup>F]N-2-(2-fluoroethoxy)benzyl)-N-(4-phenoxypyridin-3-yl)acetamide ([<sup>18</sup>F]FEPPA) (<i>n</i> = 3), [<sup>18</sup>F]fluoroacetate ([<sup>18</sup>F]FAc) (<i>n</i> = 3), and 18F-fluoro-2-deoxy-D-glucose ([<sup>18</sup>F]FDG) (<i>n</i> = 3) were obtained at 0, 1, and 2 weeks after BDL. Biochemical assays, histological assays, immunohistochemical staining assays, and next generation sequencing analyses were also performed at 0 (<i>n</i> = 3), 1 (<i>n</i> = 3), 2 (<i>n</i> = 3), and 3 (<i>n</i> = 3) weeks after BDL, which demonstrated the severe damage in rat livers after BDL. Regarding [<sup>18</sup>F]FEPPA and [<sup>18</sup>F]FDG, there was a significantly higher uptake in the liver after BDL (both <i>P</i> < 0.05), which lasted until week 2. However, the uptake of [<sup>18</sup>F]FAc in the liver was not significantly different before and after BDL (<i>P</i> = 0.28). Collectively, both [<sup>18</sup>F]FEPPA and [<sup>18</sup>F]FDG can serve as sensitive probes for detecting the liver fibrosis. However, [<sup>18</sup>F]FAc is not recommended to diagnose liver fibrosis.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"7545284"},"PeriodicalIF":4.6000,"publicationDate":"2021-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654319/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2021/7545284","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 2
Abstract
Developing sensitive diagnostic methods for a longitudinal evaluation of the status of liver fibrosis is a priority. This study is aimed at assessing the significance of longitudinal positron emission tomography (PET) imaging with 18F-labeling tracers for assessing liver fibrosis in a rat model with bile duct ligation (BDL). Twenty-one 6-week-old Sprague-Dawley male rats were used in this study. Longitudinal PET images using [18F]N-2-(2-fluoroethoxy)benzyl)-N-(4-phenoxypyridin-3-yl)acetamide ([18F]FEPPA) (n = 3), [18F]fluoroacetate ([18F]FAc) (n = 3), and 18F-fluoro-2-deoxy-D-glucose ([18F]FDG) (n = 3) were obtained at 0, 1, and 2 weeks after BDL. Biochemical assays, histological assays, immunohistochemical staining assays, and next generation sequencing analyses were also performed at 0 (n = 3), 1 (n = 3), 2 (n = 3), and 3 (n = 3) weeks after BDL, which demonstrated the severe damage in rat livers after BDL. Regarding [18F]FEPPA and [18F]FDG, there was a significantly higher uptake in the liver after BDL (both P < 0.05), which lasted until week 2. However, the uptake of [18F]FAc in the liver was not significantly different before and after BDL (P = 0.28). Collectively, both [18F]FEPPA and [18F]FDG can serve as sensitive probes for detecting the liver fibrosis. However, [18F]FAc is not recommended to diagnose liver fibrosis.
期刊介绍:
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.