The Effect of High CDC6 Levels on Predicting Poor Prognosis in Colorectal Cancer.

IF 2 4区医学 Q3 ONCOLOGY

Chemotherapy Pub Date : 2022-01-01 Epub Date: 2022-01-14 DOI:10.1159/000519913

Cheng Yang, Na Xie, Zhifei Luo, Xiling Ruan, Yixin Zhang, Weijiang Wang, Yousheng Huang

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引用次数: 2

Abstract

Introduction: We investigated the function of cell division cycle 6 (CDC6) on the prognosis in colorectal carcinoma (CRC).

Methods: CDC6 protein expression levels in 121 patients with colorectal cancer and adjacent normal mucosa were detected by immunohistochemistry.

Results: Compared to adjacent normal tissues, CDC6 mRNA level was overexpressed in CRC tissues. Moreover, CDC6 protein levels were expressed up to 93.39% (113/121) in CRC tissues in the cell nucleus or cytoplasm. However, there were only 5.79% (7/121) in normal mucosal tissues with nuclear expression. CDC6 expression was significantly correlated with TNM stage and tumor metastasis. The 5-year survival rate was lower in the high CDC6 expression group than the low group. After silencing of CDC6 expression in SW620 cells, cell proliferation was slowed, the tumor clones were decreased, and the cell cycle was arrested in G1 phase. In multivariate analysis, increased CDC6 protein expression levels in colon cancer tissues were associated with cancer metastasis, TNM stage, and patient survival time.

Conclusion: CDC6 is highly expressed in CRC, and downregulation of CDC6 can slow the growth of CRC cells in vitro. It is also an independent predictor for poor prognosis and may be a useful biomarker for targeted therapy and prognostic evaluation.

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高CDC6水平在预测结直肠癌不良预后中的作用。

前言:我们研究了细胞分裂周期6 (CDC6)在结直肠癌(CRC)预后中的作用。方法:应用免疫组织化学方法检测121例结直肠癌患者及邻近正常粘膜CDC6蛋白表达水平。结果:与邻近正常组织相比，结直肠癌组织中CDC6 mRNA水平过表达。CDC6蛋白在结直肠癌组织的细胞核或细胞质中表达量高达93.39%(113/121)。正常粘膜组织中有核表达的仅有5.79%(7/121)。CDC6表达与TNM分期及肿瘤转移有显著相关性。CDC6高表达组5年生存率低于低表达组。沉默SW620细胞CDC6表达后，细胞增殖减慢，肿瘤克隆减少，细胞周期停留在G1期。在多因素分析中，结肠癌组织中CDC6蛋白表达水平升高与肿瘤转移、TNM分期和患者生存时间相关。结论:CDC6在结直肠癌中高表达，下调CDC6可减缓体外结直肠癌细胞的生长。它也是预后不良的独立预测因子，可能是靶向治疗和预后评估的有用生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemotherapy 医学-药学

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal publishes original research articles and state-of-the-art reviews on all aspects of antimicrobial and antitumor chemotherapy. The results of experimental and clinical investigations into the microbiological and pharmacologic properties of antibacterial, antiviral and antitumor compounds are major topics of publication. Papers selected for the journal offer data concerning the efficacy, toxicology, and interactions of new drugs in single or combined applications. Studies designed to determine the pharmacokinetic and pharmacodynamics properties of similar preparations and comparing their efficacy are also included. Special emphasis is given to the development of drug-resistance, an increasing problem worldwide.